2-DE Mapping of the Blue Mussel Gill Proteome: The Usual Suspects Revisited
Abstract
:1. Introduction
2. Experimental Section
2.1. Chemicals
2.2. Animals and Sample Preparation
2.3. Gel Analysis
2.4. Mass Spectrometry and Protein Identification
3. Results and Discussion
N° | Name | Mr obs. | pI obs. | species | access number | Mr calc. | pI calc. | score | seq. | cov. | rel. Ab. | SD |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Metabolism; Carbohydrate metabolism; Amino sugar and nucleotide sugar metabolism | ||||||||||||
79 | fumarylacetoacetate hydrolase, similar | 46744 | 5.76 | Trichoplax adhaerens | gi|195998011 | 46138 | 5.89 | 85 | 2 | 5 | 0.86 | 0.08 |
125 | GDP-L-fucose synthetase | 32148 | 8.21 | Crassostrea gigas | gi|405958300 | 35147 | 6.41 | 76 | 3 | 8 | 1.77 | 0.21 |
172 | glucosamine phosphate isomerase | 24602 | 7.51 | Idiogaryops pumilis | gi|262304349 | 19748 | 5.39 | 66 | 2 | 13 | 1.91 | 0.18 |
103 | UDP-glucose 4-epimerase | 38721 | 6.31 | Crassostrea gigas | gi|405968861 | 37674 | 6.72 | 113 | 4 | 14 | 0.92 | 0.13 |
34 | UDP-N-acetylglucosamine pyrophosphorylase, provisional | 60863 | 5.97 | Capitella teleta | gi|443696999 | 57560 | 6.14 | 99 | 2 | 5 | 0.47 | 0.05 |
42 | UDP-N-acetylglucosamine pyrophosphorylase, provisional | 57755 | 6.18 | Capitella teleta | gi|443696999 | 57560 | 6.14 | 98 | 2 | 5 | 0.67 | 0.12 |
Metabolism; Carbohydrate metabolism; Glycolysis / Gluconeogenesis | ||||||||||||
74 | enolase | 49971 | 5.84 | Tomocerus sp. jcrjws1 | gi|8101744 | 41585 | 5.37 | 190 | 4 | 16 | 4.02 | 0.29 |
93 | fructose-bisphosphate aldolase | 41755 | 5.69 | Crassostrea gigas | gi|405964948 | 43741 | 5.88 | 131 | 2 | 8 | 0.87 | 0.06 |
104 | fructose-bisphosphate aldolase | 37405 | 6.34 | Mytilus edulis | gi|46909221 | 21776 | 5.86 | 186 | 4 | 23 | 0.97 | 0.07 |
122 | glyceraldehyde-3-phosphate dehydrogenase | 35098 | 8.37 | Crassostrea gigas | gi|405957058 | 36402 | 6.95 | 195 | 4 | 9 | 4.78 | 0.37 |
156 | glyceraldehyde-3-phosphate dehydrogenase A (EC 1.2.1.12) | 27219 | 6.91 | Urticina eques | gi|124264159 | 32082 | 6.51 | 70 | 2 | 8 | 3.96 | 0.18 |
37 | NADPH-dependent aldehyde reductase, putative | 60059 | 4.94 | Mytilus galloprovincialis | FL493052 | 29121 | 5.54 | 135 | 3 | 16 | 2.71 | 0.19 |
27 | phosphoenolpyruvate carboxykinase | 67990 | 7.12 | Loa loa | gi|312080904 | 72497 | 6.52 | 76 | 4 | 4 | 1.03 | 0.14 |
28 | phosphoenolpyruvate carboxykinase | 67990 | 7.22 | Loa loa | gi|312080904 | 72497 | 6.52 | 76 | 4 | 4 | 1.21 | 0.14 |
101 | phosphoglycerate kinase | 43890 | 6.85 | Caenorhabditis brenneri | gi|341896690 | 44295 | 6.28 | 291 | 6 | 18 | 2.30 | 0.31 |
84 | phosphoglycerate kinase | 43890 | 7.41 | Crassostrea gigas | gi|405963233 | 44217 | 7.59 | 85 | 4 | 13 | 1.18 | 0.11 |
163 | phosphoglycerate mutase 1 | 25755 | 4.55 | Pelodictyon phaeoclathratiforme | Q3VP85_9CHLB | 28466 | 5.20 | 72 | 2 | 8 | 2.90 | 0.38 |
159 | triosephosphate isomerase | 25844 | 6.75 | Mytilus edulis | gi|46909461 | 16417 | 4.93 | 233 | 5 | 33 | 1.88 | 0.14 |
157 | triosephosphate isomerase, partial | 25666 | 6.04 | Mytilus edulis | gi|46909461 | 16417 | 4.93 | 330 | 6 | 31 | 1.74 | 0.07 |
Metabolism; Carbohydrate metabolism; Citrate cycle (TCA cycle) | ||||||||||||
10 | aconitase 2, mitochondrial isoform 2, similar | 83797 | 6.8 | Strongylocentrotus purpuratus | gi|115735566 | 65256 | 4.96 | 105 | 3 | 6 | 0.70 | 0.07 |
11 | aconitase 2, mitochondrial isoform 2, similar | 83797 | 6.97 | Strongylocentrotus purpuratus | gi|115936456 | 84808 | 5.49 | 219 | 4 | 7 | 0.64 | 0.09 |
82 | citrate synthase, mitochondrial, predicted | 45275 | 6.49 | Strongylocentrotus purpuratus | gi|390339579 | 51662 | 6.09 | 101 | 3 | 7 | 1.67 | 0.17 |
54 | dihydrolipoamide dehydrogenase | 52991 | 6.55 | Trichoplax adhaerens | gi|196005079 | 48079 | 6.74 | 105 | 2 | 5 | 1.35 | 0.14 |
98 | isocitrate dehydrogenase | 42584 | 6.36 | Crassostrea gigas | gi|48476117 | 51365 | 8.52 | 245 | 5 | 14 | 1.17 | 0.11 |
99 | isocitrate dehydrogenase | 42584 | 6.47 | Crassostrea gigas | gi|48476117 | 51365 | 8.52 | 216 | 7 | 17 | 0.52 | 0.04 |
100 | isocitrate dehydrogenase | 42584 | 6.60 | Crassostrea gigas | gi|48476117 | 51365 | 8.52 | 445 | 9 | 20 | 2.94 | 0.21 |
161 | isocitrate dehydrogenase | 24686 | 6.88 | Mytilus trossulus | gi|385268539 | 50918 | 6.77 | 63 | 2 | 5 | 1.70 | 0.21 |
171 | isocitrate dehydrogenase | 25226 | 6.88 | Mytilus trossulus | gi|385268539 | 50918 | 6.77 | 63 | 2 | 5 | 2.21 | 0.18 |
136 | malate dehydrogenase, cytosolic | 30138 | 6.33 | Mytilus galloprovincialis | gi|73656337 | 36628 | 6.02 | 222 | 6 | 24 | 2.37 | 0.23 |
119 | malate dehydrogenase, mitochondrial | 34527 | 6.48 | Candida albicans | gi|68466091 | 34821 | 5.73 | 68 | 3 | 9 | 1.95 | 0.08 |
121 | malate dehydrogenase, mitochondrial | 34527 | 6.80 | Crassostrea gigas | gi|405963427 | 30046 | 8.20 | 64 | 2 | 7 | 0.62 | 0.06 |
118 | malate deshydrogenase, cytosolic | 35390 | 6.07 | Mytilus galloprovincialis | gi|73656337 | 36628 | 6.02 | 869 | 16 | 49 | 2.44 | 0.18 |
113 | pyruvate dehydrogenase E1 component subunit beta, mitochondrial | 32652 | 5.39 | Ascaris suum | gi|129066 | 39681 | 5.84 | 108 | 3 | 8 | 1.62 | 0.16 |
Metabolism; Carbohydrate metabolism; Pentose phosphate pathway | ||||||||||||
36 | transketolase | 62469 | 6.90 | Strongylocentrotus purpuratus | gi|336455050 | 67029 | 5.96 | 118 | 3 | 5 | 0.61 | 0.08 |
Metabolism; Energy metabolism; Transferring phosphorus-containing groups | ||||||||||||
107 | arginine kinase | 36605 | 7.29 | Macrobiotus occidentalis | gi|308199061 | 40207 | 6.91 | 89 | 2 | 8 | 2.44 | 0.17 |
108 | arginine kinase | 36605 | 7.68 | Conus novaehollandiae | gi|301341836 | 39664 | 6.34 | 123 | 2 | 3 | 3.95 | 0.43 |
Metabolism; Energy metabolism; Oxidative phosphorylation | ||||||||||||
64 | ATP synthase alpha subunit mitochondrial | 51140 | 6.84 | Crassostrea gigas | gi|405974703 | 60000 | 8.48 | 505 | 11 | 18 | 1.56 | 0.12 |
65 | ATP synthase alpha subunit mitochondrial | 50843 | 6.92 | Litopenaeus vannamei | gi|288816877 | 59416 | 8.97 | 284 | 8 | 12 | 2.50 | 0.35 |
66 | ATP synthase alpha subunit mitochondrial | 50549 | 7.11 | Pinctada fucata | gi|116008297 | 59814 | 8.92 | 764 | 14 | 23 | 3.29 | 0.31 |
87 | ATP synthase beta subunit | 46535 | 4.90 | Mytilus edulis | gi|46909261 | 46288 | 4.97 | 885 | 16 | 53 | 1.94 | 0.14 |
152 | ETF beta-like | 27504 | 6.02 | Nasonia vitripennis | gi|156543370 | 27498 | 7.66 | 236 | 6 | 19 | 1.11 | 0.07 |
67 | NADH dehydrogenase (ubiquinone) flavoprotein 1, mitochondrial | 48850 | 8.37 | Crassostrea gigas | gi|405967555 | 51955 | 8.39 | 171 | 5 | 11 | 1.01 | 0.17 |
164 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial-like | 24770 | 4.83 | Metaseiulus occidentalis | gi|391342248 | 24721 | 5.42 | 62 | 2 | 10 | 0.86 | 0.05 |
14 | NADH dehydrogenase subunit, hypothetical protein DAPPUDRAFT_192333 | 75534 | 5.38 | Daphnia pulex | gi|321476647 | 80103 | 6.00 | 162 | 3 | 4 | 0.55 | 0.05 |
15 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | 75534 | 5.33 | Crassostrea gigas | gi|405977043 | 81477 | 5.84 | 180 | 5 | 6 | 0.28 | 0.03 |
188 | nucleoside diphosphate kinase | 17020 | 7.99 | Ostrea edulis | gi|388571212 | 18860 | 6.82 | 65 | 2 | 12 | 4.98 | 0.89 |
22 | succinate dehydrogenase (ubiquinone) flavoprotein subunit | 67990 | 5.59 | Clonorchis sinensis | gi|358254399 | 72276 | 7.09 | 185 | 3 | 5 | 0.97 | 0.10 |
83 | succinate-semialdehyde dehydrogenase, mitochondrial | 45998 | 6.92 | Mytilus californianus | GE753097 | 29091 | 8.82 | 84 | 2 | 8 | 1.41 | 0.48 |
167 | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 | 24770 | 5.37 | Mytilus galloprovincialis | FL489022 | 22838 | 9.08 | 283 | 5 | 30 | 1.53 | 0.15 |
149 | voltage-dependent anion selective channel protein 2, probable | 28679 | 8.24 | Mytilus californianus | GE752193 | 23286 | 5.38 | 164 | 3 | 17 | 6.18 | 0.61 |
Metabolism; Lipid metabolism | ||||||||||||
154 | enoyl-CoA hydratase, mitochondrial-like | 27314 | 6.57 | Amphimedon queenslandica | gi|340375594 | 31912 | 5.82 | 74 | 2 | 10 | 1.27 | 0.07 |
155 | enoyl-CoA hydratase, mitochondrial-like | 27314 | 6.68 | Amphimedon queenslandica | gi|340375594 | 31912 | 5.82 | 100 | 2 | 10 | 1.54 | 0.08 |
165 | fatty acid-binding protein, provisional | 24021 | 5.06 | Mytilus galloprovincialis | FL498602 | 21271 | 8.51 | 171 | 4 | 33 | 3.69 | 0.25 |
111 | inorganic pyrophosphatase-like | 33434 | 5.17 | Mytilus californianus | ES407080 | 41244 | 8.71 | 152 | 3 | 7 | 1.63 | 0.11 |
97 | long-chain specific acyl-CoA dehydrogenase, mitochondrial precursor | 40566 | 6.17 | Homo sapiens | gi|4501857 | 48024 | 7.68 | 90 | 2 | 6 | 0.70 | 0.08 |
Metabolism; Amino acid metabolism | ||||||||||||
3 | glycine dehydrogenase | 100445 | 6.12 | Mytilus galloprovincialis | FL490887 | 29626 | 8.23 | 84 | 2 | 10 | 0.47 | 0.05 |
30 | delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial | 63776 | 5.27 | Crassostrea gigas | gi|405978465 | 64148 | 8.35 | 74 | 2 | 3 | 0.54 | 0.03 |
57 | amine oxidase, predicted | 54915 | 7.44 | Nematostella vectensis | gi|156382450 | 58581 | 6.54 | 54 | 2 | 4 | 0.76 | 0.09 |
60 | procollagen-proline dioxygenase beta subunit | 55950 | 4.62 | Mytilus galloprovincialis | gi|390979785 | 55402 | 4.53 | 449 | 13 | 25 | 7.01 | 0.42 |
95 | glutamine synthetase | 42166 | 5.98 | Tegillarca granosa | gi|306489668 | 41952 | 5.63 | 203 | 4 | 12 | 1.43 | 0.08 |
106 | cystathionine gamma-lyase | 39809 | 6.80 | Capitella teleta | gi|443685366 | 43775 | 6.14 | 78 | 2 | 4 | 1.23 | 0.08 |
123 | 3-hydroxyanthranilate 3,4-dioxygenase | 32399 | 6.70 | Suberites domuncula | gi|18076468 | 32433 | 5.57 | 70 | 2 | 5 | 0.77 | 0.07 |
Metabolism; Metabolism of other amino-acids | ||||||||||||
135 | S-formylglutathione hydrolase | 29472 | 6.25 | Acromyrmex echinatior | gi|332027837 | 18955 | 6.58 | 138 | 2 | 9 | 1.03 | 0.08 |
Metabolism; Glycan biosynthesis and metabolism | ||||||||||||
112 | short chain collagen C4, putative | 32148 | 5.20 | Mytilus galloprovincialis | EH 663252 | 32880 | 8.72 | 373 | 7 | 34 | 1.15 | 0.12 |
Metabolism; Metabolism of cofactors and vitamins; Ubiquinone and other terpenoid-quinone biosynthesis | ||||||||||||
47 | ubiquinone biosynthesis monooxygenase COQ6 | 58506 | 6.53 | Harpegnathos saltator | gi|307192550 | 52851 | 8.79 | 66 | 2 | 2 | 0.68 | 0.07 |
193 | ubiquinone biosynthesis monooxygenase COQ6 | 15255 | 7.27 | Harpegnathos saltator | gi|307192550 | 52851 | 8.79 | 59 | 2 | 2 | 4.38 | 1.35 |
Genetic Information Processing; Transcription | ||||||||||||
114 | transcriptional activator protein pur-alpha | 33170 | 5.45 | Crassostrea gigas | gi|405974727 | 27930 | 6.78 | 265 | 6 | 23 | 1.13 | 0.06 |
115 | pur-alpha, putative | 33170 | 5.55 | Ixodes scapularis | gi|242046488 | 26667 | 9.41 | 111 | 2 | 11 | 1.56 | 0.11 |
Genetic Information Processing; Translation | ||||||||||||
86 | 40S ribosomal prot SA (p 40) (34/67 kDa laminin receptor) | 43011 | 4.69 | Pinctada fucata | gi|229891605 | 33727 | 5.24 | 185 | 4 | 12 | 1.73 | 0.13 |
183 | eIF5A like | 17469 | 5.35 | Mytilus galloprovincialis | AJ516752 | 19880 | 5.23 | 226 | 5 | 38 | 6.48 | 0.79 |
51 | elongation factor 1 alpha | 56303 | 5.51 | Mytilus edulis | gi|299474235 | 50827 | 9.12 | 174 | 5 | 14 | 0.56 | 0.07 |
71 | elongation factor 1 alpha 1 | 49971 | 5.53 | Saccoglossus kowalevskii | gi|296317283 | 50711 | 9.34 | 134 | 3 | 7 | 1.75 | 0.24 |
160 | Hadh2-prov protein isoform 1, similar | 26477 | 6.91 | Strongylocentrotus purpuratus | gi|72006882 | 27479 | 6.32 | 87 | 2 | 10 | 4.13 | 0.15 |
25 | phenylalanyl-tRNA synthetase beta chain, probable | 67990 | 5.92 | Mytilus galloprovincialis | FL494288 | 25820 | 5.63 | 138 | 4 | 17 | 0.84 | 0.07 |
26 | phenylalanyl-tRNA synthetase beta chain, probable | 67990 | 6.15 | Mytilus galloprovincialis | FL494288 | 25820 | 5.63 | 99 | 2 | 9 | 0.94 | 0.12 |
46 | PRP19/PSO4 pre-mRNA processing factor 19 homolog, predicted | 57755 | 6.43 | Saccoglossus kowalevskii | gi|291228334 | 56436 | 6.60 | 78 | 2 | 6 | 0.49 | 0.04 |
199 | ribosomal protein rps12 | 13157 | 5.94 | Lineus viridis | gi|166952363 | 13852 | 8.13 | 119 | 5 | 29 | 3.39 | 0.60 |
185 | ribosomal protein rps13 | 16142 | 5.11 | Arenicola marina | gi|158187708 | 17169 | 10.59 | 74 | 2 | 17 | 1.74 | 0.16 |
198 | ribosomal protein S12 | 13258 | 5.62 | Periplaneta americana | gi|21217441 | 15585 | 5.95 | 106 | 3 | 15 | 4.24 | 0.37 |
137 | ribosomal protein S2 | 30138 | 6.48 | Chlamys farreri | gi|22203717 | 27078 | 10.49 | 147 | 5 | 26 | 0.65 | 0.07 |
Genetic Information Processing; Folding, sorting and degradation; Folding and sorting | ||||||||||||
13 | 78kDa glucose regulated protein | 75534 | 4.87 | Crassostrea gigas | gi|46359618 | 73088 | 5.02 | 567 | 11 | 16 | 3.93 | 0.36 |
68 | calreticulin, predicted | 50750 | 4.76 | Mytilus galloprovincialis | FL593839 | 27230 | 5.24 | 564 | 12 | 44 | 8.28 | 0.36 |
85 | calumenin precursor, putative | 44572 | 4.76 | Pediculus humanus corporis | gi|242005220 | 37885 | 4.61 | 65 | 2 | 3 | 3.60 | 0.20 |
38 | chaperonin | 56660 | 5.18 | Paracentrotus lividus | gi|5912574 | 62195 | 5.12 | 203 | 4 | 11 | 3.67 | 0.47 |
146 | endoplasmic reticulum protein ERp29 | 28479 | 5.85 | Crassostrea gigas | gi|405975720 | 28444 | 5.19 | 141 | 3 | 8 | 1.09 | 0.13 |
4 | glucose-regulated protein 94 | 95115 | 4.88 | Crassostrea gigas | gi|148717303 | 91795 | 4.83 | 384 | 8 | 10 | 1.60 | 0.18 |
186 | glucose-regulated protein 94 (fragment) | 16505 | 5.19 | Crassostrea gigas | gi|148717303 | 91795 | 4.83 | 101 | 2 | 3 | 1.25 | 0.08 |
20 | heat shock cognate 71 | 68990 | 5.25 | Mytilus galloprovincialis | gi|76780612 | 71508 | 5.29 | 1515 | 28 | 46 | 4.93 | 0.23 |
39 | heat shock protein 60 | 60059 | 5.23 | Biomphalaria glabrata | gi|218683627 | 31076 | 5.41 | 400 | 8 | 12 | 1.02 | 0.17 |
23 | heat shock protein 70 | 67013 | 5.64 | Mytilus galloprovincialis | gi|62989584 | 68848 | 5.35 | 90 | 3 | 6 | 0.75 | 0.09 |
24 | heat shock protein 70 | 67013 | 5.71 | Mytilus galloprovincialis | gi|62989584 | 69848 | 5.35 | 238 | 5 | 8 | 0.82 | 0.12 |
12 | heat shock protein 90 | 81772 | 5.54 | Mytilus galloprovincialis | gi|205362524 | 83358 | 4.85 | 179 | 4 | 7 | 0.45 | 0.07 |
75 | NFX1-type containing zinc finge, similar | 51140 | 5.99 | Hydra magnipapillata | gi|221116469 | 395486 | 8.08 | 59 | 3 | 0 | 3.20 | 1.15 |
143 | prohibitin | 27504 | 5.38 | Trichinella spiralis | gi|339249751 | 60213 | 6.90 | 129 | 4 | 6 | 2.40 | 0.17 |
70 | protein disulfide-isomerase, like | 50843 | 5.42 | Mytilus californianus | GE750884 | 30856 | 5.07 | 198 | 4 | 19 | 1.54 | 0.09 |
55 | protein disulfide-isomerase, predicted | 52991 | 6.64 | Trichoplax adhaerens | gi|196002337 | 52300 | 8.18 | 76 | 2 | 5 | 1.14 | 0.08 |
166 | putative small 22kd heat shock protein | 24770 | 5.35 | Mytilus californianus | ES737901 | 25707 | 5.94 | 80 | 2 | 11 | 1.39 | 0.11 |
168 | small 22kd heat shock protein, putative | 24518 | 5.49 | Mytilus californianus | ES737901 | 25707 | 5.94 | 80 | 2 | 11 | 1.98 | 0.13 |
131 | small heat shock protein 24.1 | 29692 | 5.4 | Mytilus galloprovincialis | gi|347545633 | 28691 | 5.61 | 163 | 3 | 12 | 1.42 | 0.12 |
132 | small heat shock protein 24.1 | 29582 | 5.54 | Mytilus galloprovincialis | gi|347545633 | 28691 | 5.61 | 200 | 4 | 15 | 2.34 | 0.27 |
133 | small heat shock protein 24.1 | 29582 | 5.73 | Mytilus galloprovincialis | gi|347545633 | 28691 | 5.61 | 534 | 12 | 48 | 2.57 | 0.20 |
144 | Small heat shock protein 24.1 | 28479 | 5.52 | Mytilus galloprovincialis | gi|347545633 | 28691 | 5.61 | 75 | 2 | 9 | 3.71 | 0.34 |
145 | small heat shock protein 24.1 | 28881 | 5.64 | Mytilus galloprovincialis | gi|347545633 | 28691 | 5.61 | 84 | 2 | 8 | 2.18 | 0.45 |
148 | small heat shock protein 24.1 | 28280 | 6.17 | Mytilus galloprovincialis | gi|347545633 | 28691 | 5.61 | 96 | 2 | 8 | 0.93 | 0.07 |
19 | stress-70 protein, mitochondrial, predicted mortaline-like | 67499 | 5.21 | Strongylocentrotus purpuratus | gi|72014569 | 76579 | 5.51 | 264 | 6 | 8 | 1.06 | 0.15 |
56 | TCP1 subunit epsilon like, hypothetical protein SINV_10604 | 54915 | 7.29 | Solenopsis invicta | gi|322800807 | 59845 | 5.80 | 172 | 4 | 9 | 0.77 | 0.08 |
48 | TCP1 subunit zeta | 57755 | 6.77 | Haliotis discus hannai | gi|379318220 | 58706 | 6.53 | 186 | 4 | 12 | 0.66 | 0.06 |
45 | TCP1, hypothetical protein | 58506 | 6.37 | Amblyomma maculatum | gi|346470969 | 59522 | 5.96 | 333 | 8 | 16 | 0.84 | 0.13 |
33 | TCP1, subunit beta-like | 61272 | 5.69 | Saccoglossus kowalevskii | gi|291227173 | 150827 | 8.07 | 148 | 4 | 3 | 1.05 | 0.07 |
44 | TCP1, subunit gamma isoform 1 | 60059 | 6.29 | Strongylocentrotus purpuratus | gi|115711990 | 60965 | 7.85 | 120 | 4 | 7 | 0.58 | 0.06 |
43 | TCP1, subunit eta-like isoform 1 | 55950 | 6.29 | Bombus terrestris | gi|340715736 | 60400 | 6.22 | 193 | 3 | 7 | 0.56 | 0.05 |
40 | TCP1, subunit theta | 58128 | 5.59 | Crassostrea gigas | gi|405961548 | 83831 | 5.67 | 175 | 4 | 6 | 0.70 | 0.06 |
180 | translationally controlled tumour protein | 20172 | 5.28 | Mytilus californianus | gi|359359687 | 19635 | 4.76 | 71 | 2 | 15 | 1.43 | 0.24 |
187 | tubulin-specific chaperone a-like | 16150 | 5.65 | Mytilus californianus | ES738008 | 26274 | 6.17 | 147 | 4 | 19 | 2.87 | 0.22 |
5 | valosin-containing protein-like | 93484 | 5.14 | Saccoglossus kowalevskii | gi|291242207 | 90395 | 5.18 | 296 | 6 | 10 | 0.87 | 0.10 |
Genetic Information Processing; Folding, sorting and degradation; Proteasome | ||||||||||||
76 | 26S protease regulatory subunit 6a RPT5 | 48040 | 5.09 | Crassostrea gigas | gi|405957859 | 48206 | 5.08 | 303 | 5 | 14 | 0.86 | 0.17 |
77 | 26S protease regulatory subunit 6a RPT5 | 47776 | 5.18 | Aedes aegypti | gi|157129681 | 47953 | 5.20 | 269 | 6 | 17 | 1.71 | 0.17 |
96 | 26S proteasome regulatory complex ATPase RPT4 | 42584 | 6.12 | Daphnia pulex | gi|321461635 | 44199 | 6.10 | 291 | 6 | 22 | 1.04 | 0.09 |
69 | 26S proteasome regulatory subunit T3 | 49405 | 5.21 | Schistosoma japonicum | gi|226471414 | 46930 | 5.29 | 563 | 13 | 32 | 0.85 | 0.10 |
17 | E3 ubiquitin-protein ligase TRIM33 | 75534 | 6.05 | Mytilus galloprovincialis | AJ625521 | 20697 | 7.07 | 174 | 4 | 24 | 0.26 | 0.04 |
120 | proteasome 26S subunit, non-ATPase 14-like, predicted | 33836 | 6.69 | Saccoglossus kowalevskii | gi|291239801 | 34852 | 6.07 | 89 | 3 | 9 | 0.82 | 0.09 |
142 | proteasome alpha 5 subunit-like | 27889 | 4.88 | Saccoglossus kowalevskii | gi|291243435 | 26525 | 4.74 | 268 | 4 | 22 | 1.24 | 0.07 |
169 | proteasome alpha type 2 | 24435 | 5.79 | Haliotis discus discus | gi|126697376 | 26249 | 5.73 | 173 | 3 | 18 | 1.35 | 0.10 |
177 | proteasome beta type-6 subunit | 22320 | 6.33 | Mytilus californianus | ES387982 | 30469 | 7.13 | 421 | 9 | 43 | 1.15 | 0.12 |
147 | proteasome subunit alpha type-4 | 28280 | 6.04 | Crassostrea gigas | gi|405964515 | 21464 | 5.69 | 70 | 2 | 13 | 1.26 | 0.17 |
153 | proteasome subunit alpha type-6 | 27600 | 6.12 | Crassostrea gigas | gi|405975869 | 25429 | 7.57 | 182 | 4 | 18 | 1.13 | 0.10 |
176 | ubiquination linked effector, hypothetical protein CRE_31518 | 22248 | 6.26 | Caenorhabditis remanei | gi|308460407 | 37338 | 8.82 | 57 | 1 | 2 | 0.97 | 0.08 |
Genetic Information Processing; Replication and repair | ||||||||||||
197 | histone H2B | 13942 | 5.23 | Mytilus edulis | gi|23304756 | 13781 | 10.69 | 91 | 2 | 19 | 2.35 | 0.22 |
202 | histone H4 | 12350 | 5.76 | Diprion pini | gi|1883030 | 11141 | 11.51 | 79 | 3 | 32 | 1.17 | 0.08 |
41 | meiosis-specific nuclear structural protein 1-like | 57021 | 5.91 | Saccoglossus kowalevskii | gi|291241736 | 61112 | 5.52 | 107 | 3 | 3 | 0.76 | 0.09 |
Environmental Information Processing; Signal transduction | ||||||||||||
128 | 14-3-3 epsilon protein | 29364 | 4.71 | Bombyx mori | gi|148298752 | 29767 | 4.66 | 267 | 7 | 24 | 1.55 | 0.14 |
130 | 14-3-3 epsilon protein | 29364 | 4.95 | Lepeophtheirus salmonis | gi|155966250 | 28466 | 4.67 | 102 | 3 | 8 | 3.44 | 0.27 |
129 | 14-3-3 epsilon protein | 28980 | 4.69 | Bombyx mori | gi|148298752 | 29767 | 4.66 | 214 | 7 | 24 | 2.07 | 0.28 |
174 | calcyphosin-like protein | 24505 | 5.61 | Mytilus galloprovincialis | FL489968 | 22644 | 7.00 | 141 | 2 | 10 | 1.64 | 0.28 |
18 | EF-hand domain-containing protein 1 | 72142 | 6.66 | Crassostrea gigas | gi|405964721 | 74735 | 6.23 | 126 | 4 | 5 | 1.10 | 0.08 |
Environmental Information Processing; Signaling molecules and interaction | ||||||||||||
182 | cyclophilin-type peptidyl-prolyl cis-trans isomerase-15 | 21545 | 5.95 | Mytilus galloprovincialis | FL494508 | 21737 | 5.77 | 305 | 6 | 28 | 1.22 | 0.26 |
32 | dedicator of cytokinesis protein 8, partial, predicted | 61686 | 5.57 | Amphimedon queenslandica | gi|340379755 | 236465 | 6.29 | 53 | 2 | 2 | 0.68 | 0.06 |
116 | G protein subunit beta-1 | 34110 | 5.65 | Loligo forbesii | gi|121014 | 37983 | 5.76 | 403 | 9 | 34 | 1.12 | 0.08 |
162 | GTP-binding nuclear protein Ran, provisional | 25139 | 7.99 | Crassostrea gigas | gi|405971745 | 24274 | 6.96 | 72 | 2 | 10 | 3.01 | 0.32 |
195 | peptidyl prolyl cis-trans isomerase A (II) | 14887 | 8.55 | Conus novaehollandiae | gi|289064183 | 17759 | 7.68 | 178 | 4 | 20 | 6.17 | 0.75 |
138 | receptor of Activated Kinase C 1 | 30251 | 6.96 | Mya arenaria | gi|115501910 | 35534 | 6.74 | 233 | 7 | 30 | 0.88 | 0.06 |
139 | receptor of Activated Kinase C 1 | 30251 | 7.29 | Mya arenaria | gi|115501910 | 35534 | 6.74 | 662 | 15 | 58 | 2.24 | 0.10 |
140 | receptor of Activated Kinase C 1 | 30251 | 7.41 | Mya arenaria | gi|115501910 | 35534 | 6.74 | 510 | 12 | 41 | 1.03 | 0.07 |
80 | RIB43A-like with coiled-coils protein 2 | 46744 | 6.04 | Crassostrea gigas | gi|405963849 | 45583 | 6.09 | 124 | 4 | 5 | 1.64 | 0.34 |
31 | serine/threonine-protein kinase pelle-like | 63776 | 5.44 | Bombus impatiens | gi|350396247 | 58945 | 8.87 | 54 | 1 | 1 | 1.01 | 0.10 |
102 | SET protein | 37354 | 4.49 | Crassostrea gigas | gi|405963180 | 28144 | 4.34 | 197 | 4 | 12 | 2.76 | 0.25 |
124 | sirtuin-5 | 32399 | 7.05 | Aplysia californica | gi|325197143 | 39468 | 9.03 | 117 | 2 | 6 | 0.82 | 0.07 |
Cellular Processes; Transport and catabolism | ||||||||||||
181 | C1q domain containing protein MgC1q64, putative | 20473 | 5.85 | Mytilus galloprovincialis | gi|325504427 | 24551 | 8.32 | 65 | 2 | 20 | 0.85 | 0.09 |
59 | catalase | 53914 | 7.99 | Mytilus californianus | gi|46909299 | 30345 | 6.01 | 235 | 7 | 34 | 1.31 | 0.28 |
141 | cathepsin L-like, predicted | 27219 | 4.55 | Strongylocentrotus purpuratus | gi|115715524 | 37335 | 5.14 | 64 | 2 | 3 | 2.23 | 0.23 |
16 | dipeptidyl peptidase family member 6 | 72142 | 5.68 | Crassostrea gigas | gi|405969597 | 74497 | 5.66 | 60 | 1 | 1 | 0.46 | 0.03 |
134 | dyp-type peroxidase like | 30364 | 6.00 | Trichoplax adhaerens | gi|195996389 | 33144 | 6.21 | 59 | 2 | 6 | 1.03 | 0.11 |
173 | glutathione S-transferase sigma 3 | 22964 | 5.56 | Mytilus galloprovincialis | gi|402227995 | 22940 | 5.44 | 121 | 3 | 18 | 0.65 | 0.06 |
158 | glutathione S-transferase, Class Beta | 25489 | 6.57 | Mytilus californianus | ES392983 | 38159 | 5.76 | 74 | 2 | 8 | 1.96 | 0.15 |
110 | heavy metal-binding protein HIP | 34810 | 4.92 | Mytilus edulis | gi|46395578 | 24388 | 5.09 | 165 | 6 | 45 | 2.43 | 0.38 |
105 | kin 17-mid super family, hypothetical protein AND_04962 | 38721 | 6.53 | Anopheles darlingi | gi|312382372 | 48048 | 9.44 | 55 | 2 | 5 | 0.57 | 0.04 |
58 | leucine aminopeptidase, predictive | 54915 | 8.03 | Mytilus californianus | ES400183 | 36649 | 7.01 | 332 | 8 | 40 | 2.32 | 0.25 |
6 | major vault protein | 91110 | 5.48 | Mytilus edulis | gi|5714749 | 31855 | 5.45 | 343 | 9 | 46 | 0.50 | 0.07 |
8 | major vault protein | 91892 | 5.55 | Mytilus edulis | gi|5714749 | 31855 | 5.45 | 718 | 16 | 56 | 1.27 | 0.12 |
7 | major vault protein | 91892 | 5.53 | Crassostrea gigas | gi|405974681 | 96651 | 5.58 | 73 | 2 | 2 | 0.79 | 0.08 |
9 | major vault protein | 90338 | 5.61 | Mytilus edulis | gi|5714749 | 31855 | 5.45 | 276 | 8 | 35 | 1.07 | 0.10 |
170 | peroxiredoxin | 24186 | 6.7 | Pinctada fucata | gi|306451460 | 22530 | 7.63 | 99 | 2 | 9 | 1.81 | 0.23 |
184 | peroxiredoxin V | 17924 | 6.38 | Chlamys farreri | gi|149688674 | 20431 | 8.20 | 69 | 1 | 5 | 4.55 | 0.24 |
81 | Rab GDP dissociation inhibitor alpha | 45998 | 6.21 | Schistosoma japonicum | gi|226484726 | 50623 | 6.41 | 60 | 2 | 4 | 1.32 | 0.51 |
190 | superoxide dismutase | 14887 | 5.77 | Mytilus chilensis | gi|332356353 | 15925 | 5.84 | 173 | 4 | 30 | 2.23 | 0.18 |
191 | superoxide dismutase (Cu/Zn-SOD) | 14673 | 6.11 | Mytilus edulis | gi|34481600 | 16046 | 5.84 | 289 | 4 | 31 | 3.40 | 0.22 |
175 | superoxide dismutase, mitochondrial (Mn-SOD) | 22327 | 6.00 | Mytilus galloprovincialis | gi|402122769 | 25412 | 6.44 | 124 | 2 | 9 | 1.67 | 0.15 |
203 | thioredoxin 1 | 12520 | 4.69 | Mytilus galloprovincialis | gi|391358072 | 11667 | 4.47 | 244 | 4 | 33 | 7.72 | 0.72 |
178 | thioredoxin peroxidase | 23774 | 6.70 | Cristaria plicata | gi|306451460 | 22143 | 5.95 | 75 | 2 | 10 | 1.63 | 0.16 |
21 | V-type proton ATPase catalytic subunit A | 72142 | 5.45 | Crassostrea gigas | gi|405950221 | 71148 | 5.21 | 314 | 7 | 11 | 0.69 | 0.06 |
Cellular Processes; Cell motility; Cytoskeleton proteins | ||||||||||||
92 | actin | 43011 | 5.60 | Mytilus sp. | gi|120564812 | 35392 | 5.26 | 93 | 3 | 14 | 0.90 | 0.10 |
189 | actin | 15106 | 5.15 | Schistosoma japonicum | gi|257215973 | 10215 | 5.40 | 273 | 6 | 55 | 3.99 | 0.45 |
196 | actin | 14330 | 5.05 | Hydroides elegans | gi|73532714 | 41520 | 5.39 | 357 | 9 | 19 | 2.90 | 0.28 |
88 | actin 2 = cytoplasmic actin = beta actin | 42200 | 5.22 | Crassostrea gigas | gi|18565104 | 42002 | 5.30 | 669 | 15 | 47 | 5.83 | 0.44 |
89 | actin 2 = cytoplasmic actin = beta actin | 42200 | 5.31 | Aedes aegypti | gi|67782283 | 42194 | 5.30 | 648 | 14 | 49 | 15.31 | 0.97 |
91 | actin 2 = cytoplasmic actin = beta actin | 42200 | 5.4 | Mytilus sp. | gi|120564812 | 35392 | 5.26 | 454 | 11 | 51 | 1.86 | 0.13 |
151 | actin 2 = cytoplasmic actin = beta actin | 26568 | 5.49 | Crassostrea gigas | gi|18565104 | 42002 | 5.30 | 444 | 11 | 32 | 3.25 | 0.23 |
94 | actin 5 | 40566 | 5.73 | Aedes aegypti | gi|67782283 | 42194 | 5.3 | 404 | 10 | 35 | 1.06 | 0.10 |
150 | actin-87E isoform 1, similar | 26845 | 5.28 | Tribolium castaneum | gi|91078486 | 42158 | 5.29 | 419 | 10 | 36 | 5.20 | 0.59 |
2 | catchin protein | 113783 | 5.32 | Mytilus galloprovincialis | gi|6682323 | 112777 | 5.22 | 701 | 16 | 21 | 0.40 | 0.02 |
179 | centrin-3 | 19785 | 4.66 | Crassostrea gigas | gi|405964350 | 20761 | 4.58 | 139 | 4 | 22 | 2.93 | 0.21 |
192 | destrin, partial | 15330 | 6.38 | Macaca mulatta | gi|73696362 | 12274 | 8.64 | 64 | 2 | 7 | 7.79 | 0.54 |
52 | fascin | 53914 | 6.01 | Crassostrea gigas | gi|405961655 | 56081 | 6.21 | 99 | 3 | 5 | 1.29 | 0.08 |
53 | fascin-like domain protein | 53914 | 6.15 | Tetraodon nigroviridis | gi|47209051 | 106026 | 8.68 | 85 | 2 | 2 | 0.74 | 0.08 |
78 | gelsolin | 46245 | 5.61 | Suberites domuncula | gi|27528508 | 42414 | 5.23 | 115 | 2 | 7 | 2.17 | 0.09 |
194 | hypothetical protein KGM_09271 with pleckstrin homology-like domain | 14603 | 7.58 | Danaus plexippus | gi|357623784 | 110881 | 9.64 | 72 | 2 | 2 | 4.85 | 0.72 |
29 | Na(+)/H(+) exchange regulatory cofactor NHE-RF1 | 63335 | 4.83 | Mytilus galloprovincialis | FL501152 | 22127 | 4.93 | 347 | 6 | 42 | 1.39 | 0.37 |
62 | non-neuronal cytoplasmic intermediate filament protein | 56303 | 5.27 | Mytilus californianus | GE750313 | 31541 | 7.63 | 410 | 9 | 29 | 2.03 | 0.27 |
200 | profilin like | 12468 | 6.68 | Mytilus galloprovincialis | FL496207 | 20580 | 8.33 | 243 | 6 | 37 | 5.42 | 0.37 |
117 | radial spoke head protein 9, like | 32909 | 5.82 | Crassostrea gigas | gi|405959092 | 31220 | 5.20 | 118 | 3 | 8 | 1.21 | 0.22 |
1 | spectrin alpha chain | 105775 | 4.83 | Crassostrea gigas | gi|405973516 | 287684 | 4.88 | 143 | 5 | 2 | 0.44 | 0.04 |
90 | tektin 1 | 45755 | 5.36 | Crassostrea gigas | gi|405975636 | 48654 | 6.12 | 55 | 2 | 3 | 1.86 | 0.12 |
72 | tektin-2 | 49971 | 5.58 | Crassostrea gigas | gi|405950079 | 48059 | 5.71 | 172 | 6 | 18 | 2.60 | 0.14 |
73 | tektin-4 | 48307 | 5.64 | Crassostrea gigas | gi|405967050 | 52952 | 5.53 | 172 | 7 | 12 | 2.72 | 0.18 |
109 | tropomyosin | 35098 | 4.65 | Mytilus galloprovincialis | gi|6647862 | 32807 | 4.62 | 559 | 12 | 36 | 5.25 | 0.23 |
126 | tropomyosin | 30478 | 4.69 | Mytilus edulis | gi|6647862 | 32836 | 4.64 | 312 | 6 | 12 | 1.81 | 0.13 |
127 | tropomyosin | 30593 | 4.77 | Mytilus galloprovincialis | gi|6647862 | 32807 | 4.62 | 190 | 4 | 8 | 2.17 | 0.08 |
49 | tubulin alpha-1 chain | 54915 | 5.09 | Schistosoma mansoni | gi|256087763 | 50660 | 4.97 | 780 | 18 | 47 | 11.21 | 0.75 |
61 | tubulin beta chain | 51744 | 4.93 | Crassostrea gigas | gi|56603670 | 50371 | 4.79 | 705 | 15 | 37 | 31.68 | 1.30 |
63 | tubulin, beta 2C-like, predicted | 56303 | 5.40 | Saccoglossus kowalevskii | gi|291243365 | 50516 | 4.74 | 266 | 6 | 16 | 3.06 | 0.32 |
Unknown function | ||||||||||||
35 | CCDC 151 like, coiled-coil domain containing 151 | 62469 | 6.68 | Crassostrea gigas | gi|405957528 | 63895 | 6.65 | 68 | 2 | 2 | 1.21 | 0.12 |
50 | selenium-binding protein 1, partial | 57755 | 5.48 | Crassostrea gigas | gi|405971621 | 54060 | 6.11 | 56 | 2 | 2 | 0.67 | 0.08 |
201 | hypothetical protein AND_08398 | 12519 | 7.95 | Anopheles darlingi | gi|312379666 | 38819 | 8.84 | 53 | 2 | 0 | 4.58 | 0.55 |
N° | Proteoforme | cqv | ||
---|---|---|---|---|
99 | isocitrate dehydrogenase | 5.9 | <10% | |
165 | fatty acid-binding protein, provisional | 6.7 | ||
72 | tektin-2 | 6.8 | ||
192 | destrin, partial | 7.4 | ||
78 | gelsolin | 7.7 | ||
73 | tektin-4 | 7.8 | ||
157 | triosephosphate isomerase, partial | 8.0 | ||
139 | receptor of Activated Kinase C 1 | 8.2 | ||
160 | Hadh2-prov protein isoform 1, similar | 8.3 | ||
23 | heat shock protein 70 | 8.4 | ||
60 | procollagen-proline dioxygenase beta subunit | 8.4 | ||
61 | tubulin beta chain | 8.9 | ||
109 | tropomyosin | 9.0 | ||
175 | superoxide dismutase, mitochondrial (Mn-SOD) | 9.1 | ||
68 | calreticulin, predicted | 9.1 | ||
119 | malate dehydrogenase, mitochondrial | 9.2 | ||
74 | enolase | 9.5 | ||
127 | tropomyosin | 9.5 | ||
156 | glyceraldehyde-3-phosphate dehydrogenase A (EC 1.2.1.12) | 9.6 | ||
104 | fructose-bisphosphate aldolase | 9.7 | ||
144 | Small heat shock protein 24.1 | 9.9 | ||
150 | actin-87E isoform 1, similar | 10.2 | <15% | |
105 | kin 17-mid super family, hypothetical protein AND_04962 | 10.2 | ||
115 | pur-alpha, putative | 10.3 | ||
93 | fructose-bisphosphate aldolase | 10.5 | ||
155 | enoyl-CoA hydratase, mitochondrial-like | 10.6 | ||
86 | 40S ribosomal prot SA (p 40) (34/67 kDa laminin receptor) | 10.7 | ||
143 | prohibitin | 10.9 | ||
142 | proteasome alpha 5 subunit-like | 11.0 | ||
16 | dipeptidyl peptidase family member 6 | 11.0 | ||
88 | actin 2 = cytoplasmic actin= beta actin | 11.0 | ||
95 | glutamine synthetase | 11.0 | ||
106 | cystathionine gamma-lyase | 11.0 | ||
118 | malate deshydrogenase, cytosolic | 11.0 | ||
138 | receptor of Activated Kinase C 1 | 11.0 | ||
122 | glyceraldehyde-3-phosphate dehydrogenase | 11.1 | ||
20 | heat shock cognate 71 | 11.1 | ||
136 | malate dehydrogenase, cytosolic | 11.5 | ||
89 | actin 2 = cytoplasmic actin = beta actin | 11.6 | ||
202 | histone H4 | 11.8 | ||
66 | ATP synthase alpha subunit mitochondrial | 11.9 | ||
168 | small 22kd heat shock protein, putative | 12.0 | ||
30 | delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial | 12.0 | ||
96 | 26S proteasome regulatory complex ATPase RPT4 | 12.0 | ||
185 | ribosomal protein rps13 | 12.2 | ||
70 | protein disulfide-isomerase, like | 12.2 | ||
82 | citrate synthase, mitochondrial, predicted | 12.4 | ||
52 | fascin | 12.4 | ||
35 | CCDC 151 like, coiled-coil domain containing 151 | 12.4 | ||
200 | profilin like | 12.5 | ||
152 | ETF beta-like | 12.5 | ||
184 | peroxiredoxin V | 12.6 | ||
135 | S-formylglutathione hydrolase | 12.7 | ||
116 | G protein subunit beta-1 | 12.8 | ||
179 | centrin-3 | 12.8 | ||
55 | protein disulfide-isomerase, predicted | 12.9 | ||
108 | arginine kinase | 13.0 | ||
128 | 14-3-3 epsilon protein | 13.0 | ||
154 | enoyl-CoA hydratase, mitochondrial-like | 13.1 | ||
9 | major vault protein | 13.3 | ||
18 | EF-hand domain-containing protein 1 | 13.3 | ||
113 | pyruvate dehydrogenase E1 component subunit beta, mitochondrial | 13.3 | ||
203 | thioredoxin 1 | 13.3 | ||
186 | glucose-regulated protein 94 (fragment) | 13.4 | ||
141 | cathepsin L-like, predicted | 13.4 | ||
33 | TCP1, subunit beta-like | 13.4 | ||
85 | calumenin precursor, putative | 13.4 | ||
111 | inorganic pyrophosphatase-like | 13.6 | ||
147 | proteasome subunit alpha type-4 | 13.6 | ||
114 | transcriptional activator protein pur-alpha | 13.6 | ||
53 | fascin-like domain protein | 13.7 | ||
191 | superoxide dismutase (Cu/Zn-SOD) | 13.7 | ||
32 | dedicator of cytokinesis protein 8, partial, predicted | 14.0 | ||
71 | elongation factor 1 alpha 1 | 14.0 | ||
129 | 14-3-3 epsilon ptotein | 14.2 | ||
183 | eIF5A like | 14.2 | ||
40 | TCP1, subunit theta | 14.3 | ||
13 | 78kDa glucose regulated protein | 14.4 | ||
126 | tropomyosin | 14.4 | ||
84 | phosphoglycerate kinase | 14.7 | ||
90 | tektin 1 | 15.0 | <20% | |
43 | TCP1, subunit eta-like isoform 1 | 15.1 | ||
130 | 14-3-3 epsilon protein | 15.1 | ||
91 | actin 2 = cytoplasmic actin = beta actin | 15.2 | ||
25 | phenylalanyl-tRNA synthetase beta chain, probable | 15.2 | ||
190 | superoxide dismutase | 15.3 | ||
37 | NADPH-dependent aldehyde reductase, putative | 15.4 | ||
2 | catchin protein | 15.4 | ||
159 | triosephosphate isomerase | 15.5 | ||
197 | histone H2B | 15.7 | ||
76 | 26S protease regulatory subunit 6a RPT5 | 15.9 | ||
194 | hypothetical protein KGM_09271 with pleckstrin homology-like domain | 15.9 | ||
164 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial-like | 15.9 | ||
158 | glutathione S-transferase, Class Beta | 16.0 | ||
67 | NADH dehydrogenase (ubiquinone) flavoprotein 1, mitochondrial | 16.0 | ||
140 | receptor of Activated Kinase C 1 | 16.0 | ||
62 | non-neuronal cytoplasmic intermediate filament protein | 16.1 | ||
188 | nucleoside diphosphate kinase | 16.1 | ||
87 | ATP synthase beta subunit | 16.2 | ||
151 | actin 2 = cytoplasmic actin = beta actin | 16.5 | ||
132 | small heat shock protein 24.1 | 16.6 | ||
107 | arginine kinase | 16.9 | ||
94 | actin 5 | 16.9 | ||
8 | major vault protein | 16.9 | ||
195 | peptidyl prolyl cis-trans isomerase A (II) | 16.9 | ||
4 | glucose-regulated protein 94 | 17.0 | ||
46 | PRP19/PSO4 pre-mRNA processing factor 19 homolog, predicted | 17.0 | ||
10 | aconitase 2, mitochondrial isoform 2, similar | 17.0 | ||
7 | major vault protein | 17.1 | ||
121 | malate dehydrogenase, mitochondrial | 17.2 | ||
173 | glutathione S-transferase sigma 3 | 17.3 | ||
187 | tubulin-specific chaperone a-like | 17.4 | ||
65 | ATP synthase alpha subunit mitochondrial | 17.4 | ||
14 | NADH dehydrogenase subunit, hypothetical protein DAPPUDRAFT_192333 | 17.5 | ||
79 | fumarylacetoacetate hydrolase, similar | 17.5 | ||
64 | ATP synthase alpha subunit mitochondrial | 17.9 | ||
123 | 3-hydroxyanthranilate 3,4-dioxygenase | 17.9 | ||
117 | radial spoke head protein 9, like | 17.9 | ||
153 | proteasome subunit alpha type-6 | 18.0 | ||
59 | catalase | 18.1 | ||
80 | RIB43A-like with coiled-coils protein 2 | 18.2 | ||
189 | actin | 18.2 | ||
44 | TCP1, subunit gamma isoform 1 | 18.4 | ||
100 | isocitrate dehydrogenase | 18.4 | ||
48 | TCP1 subunit zeta | 18.4 | ||
171 | isocitrate dehydrogenase | 18.5 | ||
3 | glycine dehydrogenase | 18.5 | ||
177 | proteasome beta type-6 subunit | 18.5 | ||
178 | thioredoxin peroxidase | 18.5 | ||
45 | TCP1, hypothetical protein | 18.7 | ||
131 | small heat shock protein 24.1 | 19.2 | ||
69 | 26S proteasome regulatory subunit T3 | 19.3 | ||
21 | V-type proton ATPase catalytic subunit A | 19.3 | ||
133 | small heat shock protein 24.1 | 19.3 | ||
149 | voltage-dependent anion selective channel protein 2, probable | 19.6 | ||
102 | SET protein | 19.6 | ||
12 | heat shock protein 90 | 19.6 | ||
41 | meiosis-specific nuclear structural protein 1-like | 19.7 | ||
148 | small heat shock protein 24.1 | 19.8 | ||
174 | calcyphosin-like protein | 19.8 | ||
198 | ribosomal protein S12 | 19.9 | ||
176 | ubiquination linked effector, hypothetical protein CRE_31518 | 20.0 | <25% | |
49 | tubulin alpha-1 chain | 20.2 | ||
17 | E3 ubiquitin-protein ligase TRIM33 | 20.3 | ||
103 | UDP-glucose 4-epimerase | 20.3 | ||
77 | 26S protease regulatory subunit 6a RPT5 | 20.4 | ||
19 | stress-70 protein, mitochondrial, predicted mortaline-like | 20.6 | ||
22 | succinate dehydrogenase (ubiquinone) flavoprotein subunit | 20.6 | ||
172 | glucosamine phosphate isomerase | 20.6 | ||
27 | phosphoenolpyruvate carboxykinase | 20.6 | ||
124 | sirtuin-5 | 20.6 | ||
15 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | 20.8 | ||
169 | proteasome alpha type 2 | 20.8 | ||
199 | ribosomal protein rps12 | 20.9 | ||
134 | dyp-type peroxidase like | 21.0 | ||
98 | isocitrate dehydrogenase | 21.0 | ||
47 | ubiquinone biosynthesis monooxygenase COQ6 | 21.0 | ||
29 | Na(+)/H(+) exchange regulatory cofactor NHE-RF1 | 21.1 | ||
170 | peroxiredoxin | 21.1 | ||
196 | actin | 21.1 | ||
166 | putative small 22kd heat shock protein | 21.2 | ||
181 | C1q domain containing protein MgC1q64, putative | 21.2 | ||
162 | GTP-binding nuclear protein Ran, provisional | 21.5 | ||
57 | amine oxidase, predicted | 21.5 | ||
39 | heat shock protein 60 | 21.7 | ||
31 | serine/threonine-protein kinase pelle-like | 21.8 | ||
56 | TCP1 subunit epsilon like, hypothetical protein SINV_10604 | 22.1 | ||
36 | transketolase | 22.1 | ||
97 | long-chain specific acyl-CoA dehydrogenase, mitochondrial precursor | 22.4 | ||
63 | tubulin, beta 2C-like, predicted | 22.5 | ||
182 | cyclophilin-type peptidyl-prolyl cis-trans isomerase-15 | 22.5 | ||
112 | short chain collagen C4, putative | 22.6 | ||
1 | spectrin alpha chain | 22.8 | ||
42 | UDP-N-acetylglucosamine pyrophosphorylase, provisional | 23.1 | ||
34 | UDP-N-acetylglucosamine pyrophosphorylase, provisional | 23.2 | ||
146 | endoplasmic reticulum protein ERp29 | 23.4 | ||
193 | ubiquinone biosynthesis monooxygenase COQ6 | 23.5 | ||
28 | phosphoenolpyruvate carboxykinase | 23.8 | ||
38 | chaperonin | 24.1 | ||
167 | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 | 24.1 | ||
58 | leucine aminopeptidase, predictive | 24.6 | ||
50 | selenium-binding protein 1, partial | 24.7 | ||
5 | valosin-containing protein-like | 24.8 | ||
51 | elongation factor 1 alpha | 25.9 | <30% | |
137 | ribosomal protein S2 | 25.9 | ||
163 | phosphoglycerate mutase 1 | 25.9 | ||
92 | actin | 26.4 | ||
26 | phenylalanyl-tRNA synthetase beta chain, probable | 26.9 | ||
54 | dihydrolipoamide dehydrogenase | 27.1 | ||
101 | phosphoglycerate kinase | 27.6 | ||
201 | hypothetical protein AND_08398 | 27.7 | ||
125 | GDP-L-fucose synthetase | 28.0 | ||
145 | small heat shock protein 24.1 | 31.4 | >30% | |
24 | heat shock protein 70 | 33.5 | ||
81 | Rab GDP dissociation inhibitor alpha | 37.1 | ||
11 | aconitase 2, mitochondrial isoform 2, similar | 37.4 | ||
180 | translationally controlled tumour protein | 40.8 | ||
83 | succinate-semialdehyde dehydrogenase, mitochondrial | 43.8 | ||
75 | NFX1-type containing zinc finge, similar | 47.0 |
3.1. Transcriptional and Translational Actors
3.2. Cytoskeleton
3.3. Energetic, Carbohydrate and Amino Acid Metabolisms
3.4. Antioxidant and Defence Systems
3.5. Protein Stabilisation, Folding and Sequestration
3.6. Intracellular Protein Trafficking
3.7. Ubiquitin Proteasome System
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Rocher, B.; Bultelle, F.; Chan, P.; Foll, F.L.; Letendre, J.; Monsinjon, T.; Olivier, S.; Péden, R.; Poret, A.; Vaudry, D.; et al. 2-DE Mapping of the Blue Mussel Gill Proteome: The Usual Suspects Revisited. Proteomes 2015, 3, 3-41. https://doi.org/10.3390/proteomes3010003
Rocher B, Bultelle F, Chan P, Foll FL, Letendre J, Monsinjon T, Olivier S, Péden R, Poret A, Vaudry D, et al. 2-DE Mapping of the Blue Mussel Gill Proteome: The Usual Suspects Revisited. Proteomes. 2015; 3(1):3-41. https://doi.org/10.3390/proteomes3010003
Chicago/Turabian StyleRocher, Béatrice, Florence Bultelle, Philippe Chan, Frank Le Foll, Julie Letendre, Tiphaine Monsinjon, Stéphanie Olivier, Romain Péden, Agnès Poret, David Vaudry, and et al. 2015. "2-DE Mapping of the Blue Mussel Gill Proteome: The Usual Suspects Revisited" Proteomes 3, no. 1: 3-41. https://doi.org/10.3390/proteomes3010003