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Proteomes 2015, 3(1), 42-55; doi:10.3390/proteomes3010042

The Cutting Edge of Affinity Electrophoresis Technology

Department of Functional Molecular Science, Institute of Biomedical and Health Sciences, Hiroshima University, Kasumi 1-2-3, Hiroshima 734-8553, Japan
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Academic Editor: Jacek Wisniewski
Received: 26 January 2015 / Revised: 26 January 2015 / Accepted: 11 March 2015 / Published: 18 March 2015
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Abstract

Affinity electrophoresis is an important technique that is widely used to separate and analyze biomolecules in the fields of biology and medicine. Both quantitative and qualitative information can be gained through affinity electrophoresis. Affinity electrophoresis can be applied through a variety of strategies, such as mobility shift electrophoresis, charge shift electrophoresis or capillary affinity electrophoresis. These strategies are based on changes in the electrophoretic patterns of biological macromolecules that result from interactions or complex-formation processes that induce changes in the size or total charge of the molecules. Nucleic acid fragments can be characterized through their affinity to other molecules, for example transcriptional factor proteins. Hydrophobic membrane proteins can be identified by means of a shift in the mobility induced by a charged detergent. The various strategies have also been used in the estimation of association/disassociation constants. Some of these strategies have similarities to affinity chromatography, in that they use a probe or ligand immobilized on a supported matrix for electrophoresis. Such methods have recently contributed to profiling of major posttranslational modifications of proteins, such as glycosylation or phosphorylation. Here, we describe advances in analytical techniques involving affinity electrophoresis that have appeared during the last five years. View Full-Text
Keywords: affinity electrophoresis; capillary affinity electrophoresis; affinity-trap polyacrylamide gel electrophoresis; saccharide affinity electrophoresis; supported molecular matrix electrophoresis; phosphate affinity electrophoresis; phos-tag; posttranslational modification affinity electrophoresis; capillary affinity electrophoresis; affinity-trap polyacrylamide gel electrophoresis; saccharide affinity electrophoresis; supported molecular matrix electrophoresis; phosphate affinity electrophoresis; phos-tag; posttranslational modification
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kinoshita, E.; Kinoshita-Kikuta, E.; Koike, T. The Cutting Edge of Affinity Electrophoresis Technology. Proteomes 2015, 3, 42-55.

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