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Comparative and Quantitative Global Proteomics Approaches: An Overview
Université Lille Nord de France, Lille F-59000, France
Université d'Artois, LBHE, Lens F-62307, France
IMPRT-IFR114, Lille F-59000, France
* Author to whom correspondence should be addressed.
Received: 16 September 2013; in revised form: 8 October 2013 / Accepted: 8 October 2013 / Published: 11 October 2013
Abstract: Proteomics became a key tool for the study of biological systems. The comparison between two different physiological states allows unravelling the cellular and molecular mechanisms involved in a biological process. Proteomics can confirm the presence of proteins suggested by their mRNA content and provides a direct measure of the quantity present in a cell. Global and targeted proteomics strategies can be applied. Targeted proteomics strategies limit the number of features that will be monitored and then optimise the methods to obtain the highest sensitivity and throughput for a huge amount of samples. The advantage of global proteomics strategies is that no hypothesis is required, other than a measurable difference in one or more protein species between the samples. Global proteomics methods attempt to separate quantify and identify all the proteins from a given sample. This review highlights only the different techniques of separation and quantification of proteins and peptides, in view of a comparative and quantitative global proteomics analysis. The in-gel and off-gel quantification of proteins will be discussed as well as the corresponding mass spectrometry technology. The overview is focused on the widespread techniques while keeping in mind that each approach is modular and often recovers the other.
Keywords: proteomics; proteomics: methods; electrophoresis; proteins and peptides; isotope labelling; fluorescent dies
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Deracinois, B.; Flahaut, C.; Duban-Deweer, S.; Karamanos, Y. Comparative and Quantitative Global Proteomics Approaches: An Overview. Proteomes 2013, 1, 180-218.
Deracinois B, Flahaut C, Duban-Deweer S, Karamanos Y. Comparative and Quantitative Global Proteomics Approaches: An Overview. Proteomes. 2013; 1(3):180-218.
Deracinois, Barbara; Flahaut, Christophe; Duban-Deweer, Sophie; Karamanos, Yannis. 2013. "Comparative and Quantitative Global Proteomics Approaches: An Overview." Proteomes 1, no. 3: 180-218.