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Proteomes 2013, 1(2), 87-108; doi:10.3390/proteomes1020087
Review

Exosomal Proteome Profiling: A Potential Multi-Marker Cellular Phenotyping Tool to Characterize Hypoxia-Induced Radiation Resistance in Breast Cancer

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Received: 17 June 2013; in revised form: 16 July 2013 / Accepted: 30 July 2013 / Published: 9 August 2013
(This article belongs to the Special Issue Radiation Proteomics)
View Full-Text   |   Download PDF [913 KB, uploaded 9 August 2013]
Abstract: Radiation and drug resistance are significant challenges in the treatment of locally advanced, recurrent and metastatic breast cancer that contribute to mortality. Clinically, radiotherapy requires oxygen to generate cytotoxic free radicals that cause DNA damage and allow that damage to become fixed in the genome rather than repaired. However, approximately 40% of all breast cancers have hypoxic tumor microenvironments that render cancer cells significantly more resistant to irradiation. Hypoxic stimuli trigger changes in the cell death/survival pathway that lead to increased cellular radiation resistance. As a result, the development of noninvasive strategies to assess tumor hypoxia in breast cancer has recently received considerable attention. Exosomes are secreted nanovesicles that have roles in paracrine signaling during breast tumor progression, including tumor-stromal interactions, activation of proliferative pathways and immunosuppression. The recent development of protocols to isolate and purify exosomes, as well as advances in mass spectrometry-based proteomics have facilitated the comprehensive analysis of exosome content and function. Using these tools, studies have demonstrated that the proteome profiles of tumor-derived exosomes are indicative of the oxygenation status of patient tumors. They have also demonstrated that exosome signaling pathways are potentially targetable drivers of hypoxia-dependent intercellular signaling during tumorigenesis. This article provides an overview of how proteomic tools can be effectively used to characterize exosomes and elucidate fundamental signaling pathways and survival mechanisms underlying hypoxia-mediated radiation resistance in breast cancer.
Keywords: hypoxia; radiation; breast cancer; tumor microenvironment; exosomes; proteomics hypoxia; radiation; breast cancer; tumor microenvironment; exosomes; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Thomas, S.N.; Liao, Z.; Clark, D.; Chen, Y.; Samadani, R.; Mao, L.; Ann, D.K.; Baulch, J.E.; Shapiro, P.; Yang, A.J. Exosomal Proteome Profiling: A Potential Multi-Marker Cellular Phenotyping Tool to Characterize Hypoxia-Induced Radiation Resistance in Breast Cancer. Proteomes 2013, 1, 87-108.

AMA Style

Thomas SN, Liao Z, Clark D, Chen Y, Samadani R, Mao L, Ann DK, Baulch JE, Shapiro P, Yang AJ. Exosomal Proteome Profiling: A Potential Multi-Marker Cellular Phenotyping Tool to Characterize Hypoxia-Induced Radiation Resistance in Breast Cancer. Proteomes. 2013; 1(2):87-108.

Chicago/Turabian Style

Thomas, Stefani N.; Liao, Zhongping; Clark, David; Chen, Yangyi; Samadani, Ramin; Mao, Li; Ann, David K.; Baulch, Janet E.; Shapiro, Paul; Yang, Austin J. 2013. "Exosomal Proteome Profiling: A Potential Multi-Marker Cellular Phenotyping Tool to Characterize Hypoxia-Induced Radiation Resistance in Breast Cancer." Proteomes 1, no. 2: 87-108.

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