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J. Dev. Biol. 2018, 6(3), 15; https://doi.org/10.3390/jdb6030015

The Impact of Two Different Cold-Extruded Feeds and Feeding Regimens on Zebrafish Survival, Growth and Reproductive Performance

Fish Platform, Champalimaud Center for the Unknown, 1400-038 Lisbon, Portugal
These authors contributed equally to this work.
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Academic Editors: Przemko Tylzanowski, Lisa Maves and Mark W. Majesky
Received: 4 May 2018 / Revised: 15 June 2018 / Accepted: 19 June 2018 / Published: 21 June 2018
(This article belongs to the Special Issue Zebrafish - A Model System for Developmental Biology Study)
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Abstract

Zebrafish (Danio rerio) is one of the top model organisms used in biomedical research. Therefore, it is fundamental that zebrafish facilities continuously improve husbandry methods to provide fish with the best physiological and welfare conditions that suit each experimental purpose. Nutrition is a husbandry aspect that needs further optimization, as it greatly affects growth, reproduction, health and behaviour. Here, we have compared the impact of different feeding regimens on zebrafish survival, growth and reproductive performance. Mutant and wild-type zebrafish were raised using several combinations of two cold-extruded processed feeds—Skretting®GemmaMicro and Sparos®Zebrafeed—and one live feed (rotifers). Zebrafeed® outperformed GemmaMicro® in terms of survival rate, and embryo viability was also higher when the spawners were fed with Zebrafeed® either from larval stage or upon sexual maturation. In contrast, GemmaMicro® favoured growth, both in size and weight. The use of rotifers until 60 days post-fertilization improved survival of fish co-fed with GemmaMicro®, while delaying their growth. Zebrafeed® performance was not affected by co-feeding rotifers. Overall, we showed that different nutritional formulas affect physiological parameters, allowing for the establishment of feeding protocols adapted to the objectives of each facility. At the same time, we validated Skretting®GemmaMicro and Sparos®Zebrafeed as two commercially available feeds that are well suited for zebrafish nutrition in a laboratory environment. View Full-Text
Keywords: zebrafish; dietary regimen; rotifers; feeds; survival; growth; reproductive performance zebrafish; dietary regimen; rotifers; feeds; survival; growth; reproductive performance
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Monteiro, J.F.; Martins, S.; Farias, M.; Costa, T.; Certal, A.C. The Impact of Two Different Cold-Extruded Feeds and Feeding Regimens on Zebrafish Survival, Growth and Reproductive Performance. J. Dev. Biol. 2018, 6, 15.

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