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Biomolecules 2018, 8(3), 90; https://doi.org/10.3390/biom8030090

Numerical Simulation and FRAP Experiments Show That the Plasma Membrane Binding Protein PH-EFA6 Does Not Exhibit Anomalous Subdiffusion in Cells

Membrane Domains and Viral Assembly, IRIM, UMR 9004 CNRS—Université Montpellier, 1919, Route de Mende, 34 000 Montpellier, France
Received: 18 July 2018 / Revised: 27 August 2018 / Accepted: 28 August 2018 / Published: 5 September 2018
(This article belongs to the Special Issue Cellular Membrane Domains and Organization)
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Abstract

The fluorescence recovery after photobleaching (FRAP) technique has been used for decades to measure movements of molecules in two-dimension (2D). Data obtained by FRAP experiments in cell plasma membranes are assumed to be described through a means of two parameters, a diffusion coefficient, D (as defined in a pure Brownian model) and a mobile fraction, M. Nevertheless, it has also been shown that recoveries can be nicely fit using anomalous subdiffusion. Fluorescence recovery after photobleaching (FRAP) at variable radii has been developed using the Brownian diffusion model to access geometrical characteristics of the surrounding landscape of the molecule. Here, we performed numerical simulations of continuous time random walk (CTRW) anomalous subdiffusion and interpreted them in the context of variable radii FRAP. These simulations were compared to experimental data obtained at variable radii on living cells using the pleckstrin homology (PH) domain of the membrane binding protein EFA6 (exchange factor for ARF6, a small G protein). This protein domain is an excellent candidate to explore the structure of the interface between cytosol and plasma membrane in cells. By direct comparison of our numerical simulations to the experiments, we show that this protein does not exhibit anomalous diffusion in baby hamster kidney (BHK) cells. The non Brownian PH-EFA6 dynamics observed here are more related to spatial heterogeneities such as cytoskeleton fence effects. View Full-Text
Keywords: anomalous diffusion; fluorescence recovery after photobleaching (FRAP); numerical simulations; pleckstrin homology (PH)-domain; membrane binding anomalous diffusion; fluorescence recovery after photobleaching (FRAP); numerical simulations; pleckstrin homology (PH)-domain; membrane binding
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Favard, C. Numerical Simulation and FRAP Experiments Show That the Plasma Membrane Binding Protein PH-EFA6 Does Not Exhibit Anomalous Subdiffusion in Cells. Biomolecules 2018, 8, 90.

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