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Biomolecules 2013, 3(3), 578-596; doi:10.3390/biom3030578
Review

Decarboxylation of Pyruvate to Acetaldehyde for Ethanol Production by Hyperthermophiles

 and *
Received: 9 July 2013; in revised form: 2 August 2013 / Accepted: 15 August 2013 / Published: 21 August 2013
(This article belongs to the Special Issue Enzymes and Their Biotechnological Applications)
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Abstract: Pyruvate decarboxylase (PDC encoded by pdc) is a thiamine pyrophosphate (TPP)-containing enzyme responsible for the conversion of pyruvate to acetaldehyde in many mesophilic organisms. However, no pdc/PDC homolog has yet been found in fully sequenced genomes and proteomes of hyper/thermophiles. The only PDC activity reported in hyperthermophiles was a bifunctional, TPP- and CoA-dependent pyruvate ferredoxin oxidoreductase (POR)/PDC enzyme from the hyperthermophilic archaeon Pyrococcus furiosus. Another enzyme known to be involved in catalysis of acetaldehyde production from pyruvate is CoA-acetylating acetaldehyde dehydrogenase (AcDH encoded by mhpF and adhE). Pyruvate is oxidized into acetyl-CoA by either POR or pyruvate formate lyase (PFL), and AcDH catalyzes the reduction of acetyl-CoA to acetaldehyde in mesophilic organisms. AcDH is present in some mesophilic (such as clostridia) and thermophilic bacteria (e.g., Geobacillus and Thermoanaerobacter). However, no AcDH gene or protein homologs could be found in the released genomes and proteomes of hyperthermophiles. Moreover, no such activity was detectable from the cell-free extracts of different hyperthermophiles under different assay conditions. In conclusion, no commonly-known PDCs was found in hyperthermophiles. Instead of the commonly-known PDC, it appears that at least one multifunctional enzyme is responsible for catalyzing the non-oxidative decarboxylation of pyruvate to acetaldehyde in hyperthermophiles.
Keywords: pyruvate decarboxylase; hyperthermophiles; alcohol fermentation; alcohol dehydrogenase; pyruvate ferredoxin oxidoreductase; pyruvate; acetaldehyde; ethanol pyruvate decarboxylase; hyperthermophiles; alcohol fermentation; alcohol dehydrogenase; pyruvate ferredoxin oxidoreductase; pyruvate; acetaldehyde; ethanol
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Eram, M.S.; Ma, K. Decarboxylation of Pyruvate to Acetaldehyde for Ethanol Production by Hyperthermophiles. Biomolecules 2013, 3, 578-596.

AMA Style

Eram MS, Ma K. Decarboxylation of Pyruvate to Acetaldehyde for Ethanol Production by Hyperthermophiles. Biomolecules. 2013; 3(3):578-596.

Chicago/Turabian Style

Eram, Mohammad S.; Ma, Kesen. 2013. "Decarboxylation of Pyruvate to Acetaldehyde for Ethanol Production by Hyperthermophiles." Biomolecules 3, no. 3: 578-596.


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