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Recent Advances in Cellular Glycomic Analyses
Laboratory of Medical and Functional Glycomics, Graduate School of Advanced Life Science and Frontier Research Center for Post-Genome Science and Technology, Hokkaido University, Sapporo 001-0021, Japan
* Author to whom correspondence should be addressed.
Received: 2 December 2012; in revised form: 28 January 2013 / Accepted: 14 February 2013 / Published: 21 February 2013
Abstract: A large variety of glycans is intricately located on the cell surface, and the overall profile (the glycome, given the entire repertoire of glycoconjugate-associated sugars in cells and tissues) is believed to be crucial for the diverse roles of glycans, which are mediated by specific interactions that control cell-cell adhesion, immune response, microbial pathogenesis and other cellular events. The glycomic profile also reflects cellular alterations, such as development, differentiation and cancerous change. A glycoconjugate-based approach would therefore be expected to streamline discovery of novel cellular biomarkers. Development of such an approach has proven challenging, due to the technical difficulties associated with the analysis of various types of cellular glycomes; however, recent progress in the development of analytical methodologies and strategies has begun to clarify the cellular glycomics of various classes of glycoconjugates. This review focuses on recent advances in the technical aspects of cellular glycomic analyses of major classes of glycoconjugates, including N- and O-linked glycans, derived from glycoproteins, proteoglycans and glycosphingolipids. Articles that unveil the glycomics of various biologically important cells, including embryonic and somatic stem cells, induced pluripotent stem (iPS) cells and cancer cells, are discussed.
Keywords: cellular glycomics; N-glycans; O-glycans; glycosphingolipids; mass spectrometry; glycosaminoglycans; sample preparation
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Furukawa, J.-I.; Fujitani, N.; Shinohara, Y. Recent Advances in Cellular Glycomic Analyses. Biomolecules 2013, 3, 198-225.
Furukawa J-I, Fujitani N, Shinohara Y. Recent Advances in Cellular Glycomic Analyses. Biomolecules. 2013; 3(1):198-225.
Furukawa, Jun-ichi; Fujitani, Naoki; Shinohara, Yasuro. 2013. "Recent Advances in Cellular Glycomic Analyses." Biomolecules 3, no. 1: 198-225.