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• Aldema-Ramos, ML.
• Castell, JCarles
• Muir, ZE.
• Adzet, JMaria
• Sabe, R
• Schreyer, S
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• Aldema-Ramos, ML.
• Castell, JCarles
• Muir, ZE.
• Adzet, JMaria
• Sabe, R
• Schreyer, S
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• Aldema-Ramos, ML.
• Castell, JCarles
• Muir, ZE.
• Adzet, JMaria
• Sabe, R
• Schreyer, S

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Biomolecules 2012, 2(4), 622-634; doi:10.3390/biom2040622

Article

Decorin Content and Near Infrared Spectroscopy Analysis of Dried Collagenous Biomaterial Samples

Mila L. Aldema-Ramos ^1,* , Joan Carles Castell ² , Zerlina E. Muir ¹ , Jose Maria Adzet ² , Rosa Sabe ²  and Suzanne Schreyer ³

¹ Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid LN, Wyndmoor, PA 19038, USA ² Asociación de Investigación de la Industria de Curtidos y Anexas (AIICA). Pla de la Massa, s/n, Igualada, Spain ³ Chemometrics Applications, Thermo Fisher Scientific, Tewksbury, MA 01876, USA

* Author to whom correspondence should be addressed.

Received: 10 October 2012; in revised form: 30 November 2012 / Accepted: 3 December 2012 / Published: 14 December 2012

(This article belongs to the Special Issue DNA Damage Response)

Download PDF Full-Text [358 KB, uploaded 14 December 2012 13:27 CET]

Abstract: The efficient removal of proteoglycans, such as decorin, from the hide when processing it to leather by traditional means is generally acceptable and beneficial for leather quality, especially for softness and flexibility. A patented waterless or acetone dehydration method that can generate a product similar to leather called Dried Collagenous Biomaterial (known as BCD) was developed but has no effect on decorin removal efficiency. The Alcian Blue colorimetric technique was used to assay the sulfated glycosaminoglycan (sGAG) portion of decorin. The corresponding residual decorin content was correlated to the mechanical properties of the BCD samples and was comparable to the control leather made traditionally. The waterless dehydration and instantaneous chrome tanning process is a good eco-friendly alternative to transforming hides to leather because no additional effects were observed after examination using NIR spectroscopy and additional chemometric analysis.

Keywords: proteoglycan; decorin; sulfated glycosaminoglycan (sGAG); Alcian blue; near infrared; principal component analysis (PCA); leather quality

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