Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS
AbstractThe effect of endogenous interferences of serum in multi-targeted metabolite profiling HILIC-MS/MS analysis was investigated by studying different sample preparation procedures. A modified QuEChERS dispersive SPE protocol, a HybridSPE protocol, and a combination of liquid extraction with protein precipitation were compared to a simple protein precipitation. Evaluation of extraction efficiency and sample clean-up was performed for all methods. SPE sorbent materials tested were found to retain hydrophilic analytes together with endogenous interferences, thus additional elution steps were needed. Liquid extraction was not shown to minimise matrix effects. In general, it was observed that a balance should be reached in terms of recovery, efficient clean-up, and sample treatment time when a wide range of metabolites are analysed. A quick step for removing phospholipids prior to the determination of hydrophilic endogenous metabolites is required, however, based on the results from the applied methods, further studies are needed to achieve high recoveries for all metabolites. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 112 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Tsakelidou, E.; Virgiliou, C.; Valianou, L.; Gika, H.G.; Raikos, N.; Theodoridis, G. Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS. Metabolites 2017, 7, 13.
Tsakelidou E, Virgiliou C, Valianou L, Gika HG, Raikos N, Theodoridis G. Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS. Metabolites. 2017; 7(2):13.Chicago/Turabian Style
Tsakelidou, Elisavet; Virgiliou, Christina; Valianou, Lemonia; Gika, Helen G.; Raikos, Nikolaos; Theodoridis, Georgios. 2017. "Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS." Metabolites 7, no. 2: 13.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.