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Metabolites 2016, 6(1), 3; doi:10.3390/metabo6010003

Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis

1
Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan
2
Analytical and Measuring Instruments Division, Shimadzu Corporation, 1, Nishinokyo Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan
*
Authors to whom correspondence should be addressed.
Academic Editor: Peter Meikle
Received: 2 December 2015 / Revised: 19 December 2015 / Accepted: 4 January 2016 / Published: 12 January 2016
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Abstract

Plasma-free amino acid (PFAA) levels are a useful metric for diagnosing cancer and providing a prognosis. However, the use of analysis of PFAA levels has been limited in the veterinary medicine field. We addressed the application of liquid chromatography (LC) using a pre-column labeling technique for analysis of canine PFAA levels. This method significantly shortened the analysis time relative to conventional methods. No diurnal fluctuations were detected at 9:00 AM in most PFAA levels, and food intake increased the levels of some PFAAs, including valine, leucine, tyrosine, phenylalanine, and proline. These results indicate that LC with pre-column labeling is useful for measuring canine PFAA levels, for which time of day and interval after food intake must be taken into consideration. View Full-Text
Keywords: Plasma-free amino acid; pre-column labeling; liquid chromatography measurements; canine plasma amino acid levels; pre- and post-food intake Plasma-free amino acid; pre-column labeling; liquid chromatography measurements; canine plasma amino acid levels; pre- and post-food intake
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Azuma, K.; Hirao, Y.; Hayakawa, Y.; Murahata, Y.; Osaki, T.; Tsuka, T.; Imagawa, T.; Okamoto, Y.; Ito, N. Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis. Metabolites 2016, 6, 3.

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