Next Article in Journal
Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis
Next Article in Special Issue
Visualization of Microfloral Metabolism for Marine Waste Recycling
Previous Article in Journal
Metabolic Flux Distribution during Defatting of Steatotic Human Hepatoma (HepG2) Cells
Article Menu

Export Article

Open AccessArticle
Metabolites 2016, 6(1), 2; doi:10.3390/metabo6010002

Using Molecular Networking for Microbial Secondary Metabolite Bioprospecting

1
Scottish Association for Marine Science, Scottish Marine Institute, Oban PA37 1QA, UK
2
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G1 1XQ, UK
3
Matis, Vinlandsleið 12, Reykjavik 113, Iceland
4
Faculty of Life and Environmental Sciences, University of Iceland, 101 Reykjavik, Iceland
*
Author to whom correspondence should be addressed.
Academic Editor: Peter Meikle
Received: 23 September 2015 / Revised: 23 December 2015 / Accepted: 30 December 2015 / Published: 8 January 2016
(This article belongs to the Special Issue Marine Metabolomics)
View Full-Text   |   Download PDF [2704 KB, uploaded 8 January 2016]   |  

Abstract

The oceans represent an understudied resource for the isolation of bacteria with the potential to produce novel secondary metabolites. In particular, actinomyces are well known to produce chemically diverse metabolites with a wide range of biological activities. This study characterised spore-forming bacteria from both Scottish and Antarctic sediments to assess the influence of isolation location on secondary metabolite production. Due to the selective isolation method used, all 85 isolates belonged to the phyla Firmicutes and Actinobacteria, with the majority of isolates belonging to the genera Bacillus and Streptomyces. Based on morphology, thirty-eight isolates were chosen for chemical investigation. Molecular networking based on chemical profiles (HR-MS/MS) of fermentation extracts was used to compare complex metabolite extracts. The results revealed 40% and 42% of parent ions were produced by Antarctic and Scottish isolated bacteria, respectively, and only 8% of networked metabolites were shared between these locations, implying a high degree of biogeographic influence upon secondary metabolite production. The resulting molecular network contained over 3500 parent ions with a mass range of m/z 149–2558 illustrating the wealth of metabolites produced. Furthermore, seven fermentation extracts showed bioactivity against epithelial colon adenocarcinoma cells, demonstrating the potential for the discovery of novel bioactive compounds from these understudied locations. View Full-Text
Keywords: molecular networking; secondary metabolites; bioprospecting; bacteria; Antarctica molecular networking; secondary metabolites; bioprospecting; bacteria; Antarctica
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Purves, K.; Macintyre, L.; Brennan, D.; Hreggviðsson, G.Ó.; Kuttner, E.; Ásgeirsdóttir, M.E.; Young, L.C.; Green, D.H.; Edrada-Ebel, R.; Duncan, K.R. Using Molecular Networking for Microbial Secondary Metabolite Bioprospecting. Metabolites 2016, 6, 2.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top