Next Article in Journal
UPLC Analysis of Herbal Plant Extracts
Previous Article in Journal
LC-MS-MS Can Distinguish Between Toxic and Non-Toxic Pyrrolizidine Alkaloids in Plants
Article Menu

Article Versions

Export Article

Open AccessAbstract
Sci. Pharm. 2009, 77(Short Lectures (SL)), 174; doi:10.3797/scipharm.oephg.21.SL-07 (registering DOI)

Speeding Up Pharmaceutical U-HPLC Method Development with an Integrated, Ultrafast Automated Method Scouting Solution

1
Dionex Corporation, Solothurnerstr. 259, 4600 Olten, Switzerland
2
Dionex Corporation, Dornierstr. 4, 82110 Germering, Germany
3
Dionex Corporation, Abberdaan 114, 1046 AA Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
Download PDF [197 KB, uploaded 11 May 2017]

Abstract

HPLC method development is still considered to be one of the crucial bottlenecks that impede productivity in analytical laboratories. Due to the variety of available columns, the proper selection of the stationary phase usually represents the greatest challenge. With the progress in U-HPLC which enables generic gradient separations within 5 minutes or less, ultrafast method development techniques become possible. While conventional LC rarely supported more than one generic gradient run per hour, U-HPLC can do a multitude of chromatographic runs in the same amount of time. This allows changing the experimental approach from thoroughly designing a small number of initial experiments towards a more comprehensive screening along any possible combination of column/eluent/temperature. Thanks to the very short run times of U-HPLC, this can be accomplished within the same time frame as the conventional approach. However this screening provides a significantly broader set of results, more information on the influence of the parameters within the design space, and thus more confidence in the robustness of the new method.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

HERRMANN, H.; GHIRLANDA, S.; STEINER, F.; SWART, R. Speeding Up Pharmaceutical U-HPLC Method Development with an Integrated, Ultrafast Automated Method Scouting Solution. Sci. Pharm. 2009, 77, 174.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sci. Pharm. EISSN 2218-0532 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top