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Sci. Pharm. 2009, 77(Short Lectures (SL)), 174; doi:10.3797/scipharm.oephg.21.SL-07 (registering DOI)

Speeding Up Pharmaceutical U-HPLC Method Development with an Integrated, Ultrafast Automated Method Scouting Solution

Dionex Corporation, Solothurnerstr. 259, 4600 Olten, Switzerland
Dionex Corporation, Dornierstr. 4, 82110 Germering, Germany
Dionex Corporation, Abberdaan 114, 1046 AA Amsterdam, The Netherlands
Author to whom correspondence should be addressed.
Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
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HPLC method development is still considered to be one of the crucial bottlenecks that impede productivity in analytical laboratories. Due to the variety of available columns, the proper selection of the stationary phase usually represents the greatest challenge. With the progress in U-HPLC which enables generic gradient separations within 5 minutes or less, ultrafast method development techniques become possible. While conventional LC rarely supported more than one generic gradient run per hour, U-HPLC can do a multitude of chromatographic runs in the same amount of time. This allows changing the experimental approach from thoroughly designing a small number of initial experiments towards a more comprehensive screening along any possible combination of column/eluent/temperature. Thanks to the very short run times of U-HPLC, this can be accomplished within the same time frame as the conventional approach. However this screening provides a significantly broader set of results, more information on the influence of the parameters within the design space, and thus more confidence in the robustness of the new method.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

HERRMANN, H.; GHIRLANDA, S.; STEINER, F.; SWART, R. Speeding Up Pharmaceutical U-HPLC Method Development with an Integrated, Ultrafast Automated Method Scouting Solution. Sci. Pharm. 2009, 77, 174.

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