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Sci. Pharm. 2009, 77(Short Lectures (SL)), 175; https://doi.org/10.3797/scipharm.oephg.21.SL-08 (registering DOI)
UPLC Analysis of Herbal Plant Extracts
Waters GesmbH, A-1130 Wien, Hietzinger Hauptstraße 145, Austria
Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
Abstract UPLC (ultraperformance liquid chromatography) has become commercially available in 2004. Like other chromatographic techniques using sub two micron (STM) particles, UPLC is capable of accelerating existing HPLC methods by a factor of 10 to16. However, analyzing plant extracts, a gain in separation power is often of greater importance than a gain in speed. This requires the use of columns generating backpressures up to and exceeding 15000psi (1000bar). The theory of UPLC is briefly explained and the benefits of UPLC vs. HPLC are illustrated by several examples, incl. ginkgo, kava, black cohosh, milk thistle, and passion flower extracts.
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KUFFNER, H.U. UPLC Analysis of Herbal Plant Extracts. Sci. Pharm. 2009, 77, 175.
KUFFNER HU. UPLC Analysis of Herbal Plant Extracts. Scientia Pharmaceutica. 2009; 77(Short Lectures (SL)):175.
KUFFNER, H. U. 2009. "UPLC Analysis of Herbal Plant Extracts." Sci. Pharm. 77, Short Lectures (SL): 175.
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