Effectiveness of a Novel Compound HAIR & SCALP COMPLEX on Hair Follicle Regeneration

Round 1

Reviewer 1 Report

This manuscript describes treatment compounds that could help slow down hair loss and encourage new hair growth. It reports that exosomes combined with the bioactive molecules with regenerative potential of GF20, in HSC50+, resulted in a significant increase in bulb growth and regeneration of the dermal papilla.

The topic is very interesting and timely, the experimental design and reported results are satisfactory and the paper is well written and organized. The authors need to consider the following points in the revised manuscript:

§  The introduction is nicely written; it is very informative and sufficiently introduces the topic of the research. However, the last paragraph does not clearly show what the authors want to investigate. Here elongation or hair follicle regeneration and how? More details need to be provided. This is also applied to the abstract.

§  What do the authors mean by “The accepted size for exosomes of 30-150” ?

§  The authors need to describe GF20 in some detail in the introduction.

§  Table 1 could be displayed as one column to avoid confusion.

§  Section 2.7: “ntact and anagen phase VI 183 follicles, observed and selected by stereomicroscopy, were cultured in 24-well plates (1 per well) with 500 μl Williams E medium (Gibco BRL, Rockville, MD, USA), with insulin 185 10 μg ⁄ ml, hydrocortisone 10 ng ⁄ ml, streptomycin 10 μg ⁄ ml and penicillin 100 U ⁄ ml 186 (Wuppertal, Germany), at 37°C and 5% CO2.”

The authors need to confirm and justify why only 1 per well?

§  Section 3.2: “Plant-Derived NanoVesicles (PDNVs) of C. bergamia within the separated and purified 210 suspension showed a range of 182.5 ± 1.3 nm and a concentration of 2x1010 mL-1.” Should be an average of 182.5 ± 1.3.

§  Section 3.3: “In Figure 2 was 222 reported the concentration (pg/mL) of main bioactive substances isolated from GF20.”needs to be re-phrased.

§  Figures 5 and 6: The y-axis should be labelled.

§  Discussion: Lines 293-303: This text fits more in the introduction, not the discussion. This also applied to the lines 332-304.

Author Response

Dear Reviewer,

we thank you for the suggestions and corrections. We did all of them as we considered them useful for improving our manuscript.

Author Response File: Author Response.pdf

Reviewer 2 Report

Potential target growth factors/cytokines for hair growth stimulation are of great interest. Nowadays only PRP injections and DHT inhibitors are used to combat moderate hair loss (except hair transplantation). Therefore, characterization of specific cytokines are of great interest and this article has its value.

However, translatability of this finding is not discussed at all. This should be suggested at least in discussion - how this finding will become applicable.

Grammar and spelling should be improved. For example, results section of the abstract "The analyzes demonstrated.."

Author Response

Dear Reviewer, we thank you for the suggestions and corrections. We did all of them as we considered them useful for improving our manuscript.

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

Authors corrected suggested changes. Thus, manuscript can be accepted.