Next Article in Journal / Special Issue
In Vivo Non-Invasive Evaluation of Actinic Keratoses Response to Methyl-Aminolevulinate-Photodynamic Therapy (MAL-PDT) by Reflectance Confocal Microscopy
Previous Article in Journal
Bio-Guided Targeting for Preservative and Anti-Ageing Cosmetic Ingredient Development
Cosmetics 2014, 1(1), 29-36; doi:10.3390/cosmetics1010029
Article

In Vivo Cosmetic Product Efficacy Testing by Analyzing Epidermal Proteins Extracted from Tape Strips

1,* , 1
, 2
, 2
, 1
, 2
, 1
 and 1
Received: 30 December 2013; in revised form: 25 January 2014 / Accepted: 27 January 2014 / Published: 14 February 2014
(This article belongs to the Special Issue Selected Papers from ISBS/SICC 1st Joint International Congress)
View Full-Text   |   Download PDF [279 KB, uploaded 14 February 2014]   |   Browse Figure
Abstract: The objective of this in vivo pilot study was to investigate whether differential biomarker analysis from skin tape strips could be used, not only to evaluate the difference between treated and untreated skin, but also to evaluate the effect of different product treatments. Ten volunteers were included in the study, applying two different basic formulations on their forearms. After four weeks of product application, and also after one week of treatment remission, tape strips were collected from the different treatment sites, as well as from untreated skin. The biomarkers investigated were selected to cover different aspects of epidermal differentiation and in connection with moisturization and barrier function. Levels of Involucrin were increased in both treatments, compared to untreated skin, whereas the levels of Keratin-6 were decreased for both treatments. In addition, a pattern for increased levels of Hornerin and Claudin-1 was also detected. There were no significant differences between the two treatments, only for treatment compared to untreated, but there were tendencies for different effect on some of the biomarkers investigated, differences that may reach significance with increased sample size. The major differences between the two treatments in this study were seen after one week of product remission, although due to too small sample size these differences were not significant.
Keywords: tape stripping; Keratin; Involucrin; Claudin-1; Hornerin tape stripping; Keratin; Involucrin; Claudin-1; Hornerin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Westman, M.; Al-Bader, T.; Merinville, E.; Cattley, K.; Lafon-Kolb, V.; Darbon, J.; Mavon, A.; Laloeuf, A. In Vivo Cosmetic Product Efficacy Testing by Analyzing Epidermal Proteins Extracted from Tape Strips. Cosmetics 2014, 1, 29-36.

AMA Style

Westman M, Al-Bader T, Merinville E, Cattley K, Lafon-Kolb V, Darbon J, Mavon A, Laloeuf A. In Vivo Cosmetic Product Efficacy Testing by Analyzing Epidermal Proteins Extracted from Tape Strips. Cosmetics. 2014; 1(1):29-36.

Chicago/Turabian Style

Westman, Marie; Al-Bader, Tamara; Merinville, Eve; Cattley, Kevin; Lafon-Kolb, Virginie; Darbon, Josephine; Mavon, Alain; Laloeuf, Aurelie. 2014. "In Vivo Cosmetic Product Efficacy Testing by Analyzing Epidermal Proteins Extracted from Tape Strips." Cosmetics 1, no. 1: 29-36.

Cosmetics EISSN 2079-9284 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert