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A Versatile Tool for Stable Inhibition of microRNA Activity
Institute of Biomedical Technologies, National Research Council, 20090 Segrate-Milan, Italy
Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Robert-Kock Str. 39, D-50931, Cologne, Germany
Pathology and Anatomy Unit, Manerbio, Desenzano d/G, 25025 Manerbio, Italy
University of Münster Medical Center, Gynecology and Obstetrics, Münster 48149, Germany
* Authors to whom correspondence should be addressed.
Received: 11 April 2013; in revised form: 13 May 2013 / Accepted: 15 May 2013 / Published: 28 June 2013
Abstract: MicroRNAs (miRNAs) are a class of small RNAs (18–22 nt) that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing. As aberrant expression of miRNAs is implicated in important diseases including cancer miRNA-based therapies are under intensive investigation. We optimized strategies to stably or conditionally generate miRNA inhibitors for a continuous block of miRNA activity that allows for probing miRNA function in long-term cell culture experiments, cancer xenografts, 3D tissue models and for in vivo studies with transgenic organisms.
Keywords: miRNA; lentivirus; miR-inhibitor
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Pelucchi, P.; Tria, V.; Martino, V.; Sabour, D.; Bertalot, G.; Molgora, S.; Palizban, M.; Götte, M.; Zucchi, I.; Reinbold, R.A. A Versatile Tool for Stable Inhibition of microRNA Activity. Biology 2013, 2, 861-871.
Pelucchi P, Tria V, Martino V, Sabour D, Bertalot G, Molgora S, Palizban M, Götte M, Zucchi I, Reinbold RA. A Versatile Tool for Stable Inhibition of microRNA Activity. Biology. 2013; 2(3):861-871.
Pelucchi, Paride; Tria, Valeria; Martino, Valentina; Sabour, Davood; Bertalot, Giovanni; Molgora, Stefano; Palizban, Mira; Götte, Martin; Zucchi, Ileana; Reinbold, Rolland A. 2013. "A Versatile Tool for Stable Inhibition of microRNA Activity." Biology 2, no. 3: 861-871.