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Biology 2013, 2(3), 861-871; doi:10.3390/biology2030861
Article

A Versatile Tool for Stable Inhibition of microRNA Activity

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Received: 11 April 2013; in revised form: 13 May 2013 / Accepted: 15 May 2013 / Published: 28 June 2013
(This article belongs to the Special Issue Gene Expression and Regulation)
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Abstract: MicroRNAs (miRNAs) are a class of small RNAs (18–22 nt) that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing. As aberrant expression of miRNAs is implicated in important diseases including cancer miRNA-based therapies are under intensive investigation. We optimized strategies to stably or conditionally generate miRNA inhibitors for a continuous block of miRNA activity that allows for probing miRNA function in long-term cell culture experiments, cancer xenografts, 3D tissue models and for in vivo studies with transgenic organisms.
Keywords: miRNA; lentivirus; miR-inhibitor miRNA; lentivirus; miR-inhibitor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pelucchi, P.; Tria, V.; Martino, V.; Sabour, D.; Bertalot, G.; Molgora, S.; Palizban, M.; Götte, M.; Zucchi, I.; Reinbold, R.A. A Versatile Tool for Stable Inhibition of microRNA Activity. Biology 2013, 2, 861-871.

AMA Style

Pelucchi P, Tria V, Martino V, Sabour D, Bertalot G, Molgora S, Palizban M, Götte M, Zucchi I, Reinbold RA. A Versatile Tool for Stable Inhibition of microRNA Activity. Biology. 2013; 2(3):861-871.

Chicago/Turabian Style

Pelucchi, Paride; Tria, Valeria; Martino, Valentina; Sabour, Davood; Bertalot, Giovanni; Molgora, Stefano; Palizban, Mira; Götte, Martin; Zucchi, Ileana; Reinbold, Rolland A. 2013. "A Versatile Tool for Stable Inhibition of microRNA Activity." Biology 2, no. 3: 861-871.



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