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Biology 2013, 2(3), 861-871; doi:10.3390/biology2030861

A Versatile Tool for Stable Inhibition of microRNA Activity

1
Institute of Biomedical Technologies, National Research Council, 20090 Segrate-Milan, Italy
2
Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Robert-Kock Str. 39, D-50931, Cologne, Germany
3
Pathology and Anatomy Unit, Manerbio, Desenzano d/G, 25025 Manerbio, Italy
4
University of Münster Medical Center, Gynecology and Obstetrics, Münster 48149, Germany
*
Authors to whom correspondence should be addressed.
Received: 11 April 2013 / Revised: 13 May 2013 / Accepted: 15 May 2013 / Published: 28 June 2013
(This article belongs to the Special Issue Gene Expression and Regulation)
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Abstract

MicroRNAs (miRNAs) are a class of small RNAs (18–22 nt) that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing. As aberrant expression of miRNAs is implicated in important diseases including cancer miRNA-based therapies are under intensive investigation. We optimized strategies to stably or conditionally generate miRNA inhibitors for a continuous block of miRNA activity that allows for probing miRNA function in long-term cell culture experiments, cancer xenografts, 3D tissue models and for in vivo studies with transgenic organisms.
Keywords: miRNA; lentivirus; miR-inhibitor miRNA; lentivirus; miR-inhibitor
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Pelucchi, P.; Tria, V.; Martino, V.; Sabour, D.; Bertalot, G.; Molgora, S.; Palizban, M.; Götte, M.; Zucchi, I.; Reinbold, R.A. A Versatile Tool for Stable Inhibition of microRNA Activity. Biology 2013, 2, 861-871.

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