Biology 2013, 2(1), 177-188; doi:10.3390/biology2010177
Article

Novel Cold-Adapted Esterase MHlip from an Antarctic Soil Metagenome

1,2,†,* email, 1,†email, 1email, 1email, 3email, 4email, 1email and 1,5email
Received: 3 December 2012; in revised form: 4 January 2013 / Accepted: 11 January 2013 / Published: 25 January 2013
(This article belongs to the Special Issue Polar Microbiology: Recent Advances and Future Perspectives)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: An Antarctic soil metagenomic library was screened for lipolytic enzymes and allowed for the isolation of a new cytosolic esterase from the a/b hydrolase family 6, named MHlip. This enzyme is related to hypothetical genes coding esterases, aryl-esterases and peroxydases, among others. MHlip was produced, purified and its activity was determined. The substrate profile of MHlip reveals a high specificity for short p-nitrophenyl-esters. The apparent optimal activity of MHlip was measured for p-nitrophenyl-acetate, at 33 °C, in the pH range of 6–9. The MHlip thermal unfolding was investigated by spectrophotometric methods, highlighting a transition (Tm) at 50 °C. The biochemical characterization of this enzyme showed its adaptation to cold temperatures, even when it did not present evident signatures associated with cold-adapted proteins. Thus, MHlip adaptation to cold probably results from many discrete structural modifications, allowing the protein to remain active at low temperatures. Functional metagenomics is a powerful approach to isolate new enzymes with tailored biophysical properties (e.g., cold adaptation). In addition, beside the ever growing amount of sequenced DNA, the functional characterization of new catalysts derived from environment is still required, especially for poorly characterized protein families like α/b hydrolases.
Keywords: α/b hydrolase; lipolytic enzymes; metagenomics; p-nitrophenyl-ester; cold-adaptation
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MDPI and ACS Style

Berlemont, R.; Jacquin, O.; Delsaute, M.; La Salla, M.; Georis, J.; Verté, F.; Galleni, M.; Power, P. Novel Cold-Adapted Esterase MHlip from an Antarctic Soil Metagenome. Biology 2013, 2, 177-188.

AMA Style

Berlemont R, Jacquin O, Delsaute M, La Salla M, Georis J, Verté F, Galleni M, Power P. Novel Cold-Adapted Esterase MHlip from an Antarctic Soil Metagenome. Biology. 2013; 2(1):177-188.

Chicago/Turabian Style

Berlemont, Renaud; Jacquin, Olivier; Delsaute, Maud; La Salla, Marcello; Georis, Jacques; Verté, Fabienne; Galleni, Moreno; Power, Pablo. 2013. "Novel Cold-Adapted Esterase MHlip from an Antarctic Soil Metagenome." Biology 2, no. 1: 177-188.

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