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Biology 2013, 2(1), 189-205; doi:10.3390/biology2010189
Review

MicroRNA Target Identification—Experimental Approaches

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Received: 29 October 2012 / Revised: 19 December 2012 / Accepted: 24 December 2012 / Published: 25 January 2013
View Full-Text   |   Download PDF [198 KB, 28 January 2013; original version 25 January 2013]

Abstract

MicroRNAs (miRNAs) are small non-coding RNA molecules of 21–23 nucleotides that control gene expression at the post-transcriptional level. They have been shown to play a vital role in a wide variety of biological processes and dysregulated expression of miRNAs is observed in many pathologies. Understanding the mechanism of action and identifying functionally important mRNA targets of a specific miRNA are essential to unravelling its biological function and to assist miRNA-based drug development. This review summarizes the current understanding of the mechanistic aspects of miRNA-mediated gene repression and focuses on the different approaches for miRNA target identification that have been proposed in recent years.
Keywords: miRNA; gene expression; miRISC; miRNA target identification; RIP-Seq; HITS-CLIP; HT-Seq miRNA; gene expression; miRISC; miRNA target identification; RIP-Seq; HITS-CLIP; HT-Seq
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Martinez-Sanchez, A.; Murphy, C.L. MicroRNA Target Identification—Experimental Approaches. Biology 2013, 2, 189-205.

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