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Biology 2013, 2(1), 189-205; doi:10.3390/biology2010189
Review

MicroRNA Target Identification—Experimental Approaches

 and
*
Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, 65 Aspenlea Road, London W6 8LH, UK
* Author to whom correspondence should be addressed.
Received: 29 October 2012 / Revised: 19 December 2012 / Accepted: 24 December 2012 / Published: 25 January 2013
View Full-Text   |   Download PDF [198 KB, 28 January 2013; original version 25 January 2013]

Abstract

MicroRNAs (miRNAs) are small non-coding RNA molecules of 21–23 nucleotides that control gene expression at the post-transcriptional level. They have been shown to play a vital role in a wide variety of biological processes and dysregulated expression of miRNAs is observed in many pathologies. Understanding the mechanism of action and identifying functionally important mRNA targets of a specific miRNA are essential to unravelling its biological function and to assist miRNA-based drug development. This review summarizes the current understanding of the mechanistic aspects of miRNA-mediated gene repression and focuses on the different approaches for miRNA target identification that have been proposed in recent years.
Keywords: miRNA; gene expression; miRISC; miRNA target identification; RIP-Seq; HITS-CLIP; HT-Seq miRNA; gene expression; miRISC; miRNA target identification; RIP-Seq; HITS-CLIP; HT-Seq
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Martinez-Sanchez, A.; Murphy, C.L. MicroRNA Target Identification—Experimental Approaches. Biology 2013, 2, 189-205.

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