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Biology 2013, 2(1), 189-205; doi:10.3390/biology2010189
Review

MicroRNA Target Identification—Experimental Approaches

 and *
Received: 29 October 2012; in revised form: 19 December 2012 / Accepted: 24 December 2012 / Published: 25 January 2013
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Abstract: MicroRNAs (miRNAs) are small non-coding RNA molecules of 21–23 nucleotides that control gene expression at the post-transcriptional level. They have been shown to play a vital role in a wide variety of biological processes and dysregulated expression of miRNAs is observed in many pathologies. Understanding the mechanism of action and identifying functionally important mRNA targets of a specific miRNA are essential to unravelling its biological function and to assist miRNA-based drug development. This review summarizes the current understanding of the mechanistic aspects of miRNA-mediated gene repression and focuses on the different approaches for miRNA target identification that have been proposed in recent years.
Keywords: miRNA; gene expression; miRISC; miRNA target identification; RIP-Seq; HITS-CLIP; HT-Seq miRNA; gene expression; miRISC; miRNA target identification; RIP-Seq; HITS-CLIP; HT-Seq
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Martinez-Sanchez, A.; Murphy, C.L. MicroRNA Target Identification—Experimental Approaches. Biology 2013, 2, 189-205.

AMA Style

Martinez-Sanchez A, Murphy CL. MicroRNA Target Identification—Experimental Approaches. Biology. 2013; 2(1):189-205.

Chicago/Turabian Style

Martinez-Sanchez, Aida; Murphy, Chris L. 2013. "MicroRNA Target Identification—Experimental Approaches." Biology 2, no. 1: 189-205.


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