Biology 2013, 2(1), 189-205; doi:10.3390/biology2010189
Review

MicroRNA Target Identification—Experimental Approaches

Received: 29 October 2012; in revised form: 19 December 2012 / Accepted: 24 December 2012 / Published: 25 January 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: MicroRNAs (miRNAs) are small non-coding RNA molecules of 21–23 nucleotides that control gene expression at the post-transcriptional level. They have been shown to play a vital role in a wide variety of biological processes and dysregulated expression of miRNAs is observed in many pathologies. Understanding the mechanism of action and identifying functionally important mRNA targets of a specific miRNA are essential to unravelling its biological function and to assist miRNA-based drug development. This review summarizes the current understanding of the mechanistic aspects of miRNA-mediated gene repression and focuses on the different approaches for miRNA target identification that have been proposed in recent years.
Keywords: miRNA; gene expression; miRISC; miRNA target identification; RIP-Seq; HITS-CLIP; HT-Seq
PDF Full-text Download PDF Full-Text [198 KB, Updated Version, uploaded 28 January 2013 16:15 CET]
The original version is still available [198 KB, uploaded 25 January 2013 14:01 CET]

Export to BibTeX |
EndNote


MDPI and ACS Style

Martinez-Sanchez, A.; Murphy, C.L. MicroRNA Target Identification—Experimental Approaches. Biology 2013, 2, 189-205.

AMA Style

Martinez-Sanchez A, Murphy CL. MicroRNA Target Identification—Experimental Approaches. Biology. 2013; 2(1):189-205.

Chicago/Turabian Style

Martinez-Sanchez, Aida; Murphy, Chris L. 2013. "MicroRNA Target Identification—Experimental Approaches." Biology 2, no. 1: 189-205.

Biology EISSN 2079-7737 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert