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Antibiotics 2017, 6(4), 33; doi:10.3390/antibiotics6040033

Dual Regulation of the Small RNA MicC and the Quiescent Porin OmpN in Response to Antibiotic Stress in Escherichia coli

UMR_MD1, Aix-Marseille Univ & Institut de Recherche Biomédicale des Armées, 27 Boulevard Jean Moulin, 13005 Marseille, France
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Author to whom correspondence should be addressed.
Academic Editor: Leonard Amaral
Received: 27 October 2017 / Revised: 27 November 2017 / Accepted: 3 December 2017 / Published: 6 December 2017
(This article belongs to the Special Issue Top 35 of Antibiotics Travel Awards 2017)
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Abstract

Antibiotic resistant Gram-negative bacteria are a serious threat for public health. The permeation of antibiotics through their outer membrane is largely dependent on porin, changes in which cause reduced drug uptake and efficacy. Escherichia coli produces two major porins, OmpF and OmpC. MicF and MicC are small non-coding RNAs (sRNAs) that modulate the expression of OmpF and OmpC, respectively. In this work, we investigated factors that lead to increased production of MicC. micC promoter region was fused to lacZ, and the reporter plasmid was transformed into E. coli MC4100 and derivative mutants. The response of micC–lacZ to antimicrobials was measured during growth over a 6 h time period. The data showed that the expression of micC was increased in the presence of β-lactam antibiotics and in an rpoE depleted mutant. Interestingly, the same conditions enhanced the activity of an ompN–lacZ fusion, suggesting a dual transcriptional regulation of micC and the quiescent adjacent ompN. Increased levels of OmpN in the presence of sub-inhibitory concentrations of chemicals could not be confirmed by Western blot analysis, except when analyzed in the absence of the sigma factor σE. We suggest that the MicC sRNA acts together with the σE envelope stress response pathway to control the OmpC/N levels in response to β-lactam antibiotics. View Full-Text
Keywords: Escherichia coli; outer membrane porins; regulatory small RNAs; membrane transport; antibiotic susceptibility Escherichia coli; outer membrane porins; regulatory small RNAs; membrane transport; antibiotic susceptibility
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MDPI and ACS Style

Dam, S.; Pagès, J.-M.; Masi, M. Dual Regulation of the Small RNA MicC and the Quiescent Porin OmpN in Response to Antibiotic Stress in Escherichia coli. Antibiotics 2017, 6, 33.

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