Pectocin M1 (PcaM1) Inhibits Escherichia coli Cell Growth and Peptidoglycan Biosynthesis through Periplasmic Expression
AbstractColicins are bacterial toxins produced by some Escherichia coli strains. They exhibit either enzymatic or pore-forming activity towards a very limited number of bacterial species, due to the high specificity of their reception and translocation systems. Yet, we succeeded in making the colicin M homologue from Pectobacterium carotovorum, pectocin M1 (PcaM1), capable of inhibiting E. coli cell growth by bypassing these reception and translocation steps. This goal was achieved through periplasmic expression of this pectocin. Indeed, when appropriately addressed to the periplasm of E. coli, this pectocin could exert its deleterious effects, i.e., the enzymatic degradation of the peptidoglycan lipid II precursor, which resulted in the arrest of the biosynthesis of this essential cell wall polymer, dramatic morphological changes and, ultimately, cell lysis. This result leads to the conclusion that colicin M and its various orthologues constitute powerful antibacterial molecules able to kill any kind of bacterium, once they can reach their lipid II target. They thus have to be seriously considered as promising alternatives to antibiotics. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 14241 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Chérier, D.; Giacomucci, S.; Patin, D.; Bouhss, A.; Touzé, T.; Blanot, D.; Mengin-Lecreulx, D.; Barreteau, H. Pectocin M1 (PcaM1) Inhibits Escherichia coli Cell Growth and Peptidoglycan Biosynthesis through Periplasmic Expression. Antibiotics 2016, 5, 36.
Chérier D, Giacomucci S, Patin D, Bouhss A, Touzé T, Blanot D, Mengin-Lecreulx D, Barreteau H. Pectocin M1 (PcaM1) Inhibits Escherichia coli Cell Growth and Peptidoglycan Biosynthesis through Periplasmic Expression. Antibiotics. 2016; 5(4):36.Chicago/Turabian Style
Chérier, Dimitri; Giacomucci, Sean; Patin, Delphine; Bouhss, Ahmed; Touzé, Thierry; Blanot, Didier; Mengin-Lecreulx, Dominique; Barreteau, Hélène. 2016. "Pectocin M1 (PcaM1) Inhibits Escherichia coli Cell Growth and Peptidoglycan Biosynthesis through Periplasmic Expression." Antibiotics 5, no. 4: 36.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.