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Biosensors 2018, 8(2), 36; doi:10.3390/bios8020036

The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs

1
Faculdade de Farmácia, Universidade Federal do Goiás (UFG), rua 221 esquina com a 5ª avenida s/n, Setor Universitário, Goiânia-GO 74605-170, Brazil
2
Department of Chemistry, Faculty of Applied Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa
*
Authors to whom correspondence should be addressed.
Received: 20 February 2018 / Revised: 12 March 2018 / Accepted: 29 March 2018 / Published: 2 April 2018
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Abstract

The vegetable kingdom is a wide source of a diverse variety of enzymes with broad biotechnological applications. Among the main classes of plant enzymes, the polyphenol oxidases, which convert phenolic compounds to the related quinones, have been successfully used for biosensor development. The oxidation products from such enzymes can be electrochemically reduced, and the sensing is easily achieved by amperometric transducers. In this work, the polyphenoloxidases were extracted from jurubeba (Solanum paniculatum L.) fruits, and the extract was used to construct a carbon paste-based biosensor for pharmaceutical analysis and applications. The assay optimization was performed using a 0.1 mM catechol probe, taking into account the amount of enzymatic extract (50 or 200 μL) and the optimum pH (3.0 to 9.0) as well as some electrochemical differential pulse voltammetric (DPV) parameters (e.g., pulse amplitude, pulse range, pulse width, scan rate). Under optimized conditions, the biosensor was evaluated for the quantitative determination of acetaminophen, acetylsalicylic acid, methyldopa, and ascorbic acid. The best performance was obtained for acetaminophen, which responded linearly in the range between 5 and 245 μM (R = 0.9994), presenting a limit of detection of 3 μM and suitable repeatability ranging between 1.52% and 1.74% relative standard deviation (RSD). View Full-Text
Keywords: plant enzymes; vegetable polyphenoloxidases; amperometric biosensors; pharmaceutical analysis plant enzymes; vegetable polyphenoloxidases; amperometric biosensors; pharmaceutical analysis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Antunes, R.S.; Garcia, L.F.; Somerset, V.S.; Gil, E.S.; Lopes, F.M. The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs. Biosensors 2018, 8, 36.

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