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Biosensors 2018, 8(1), 19; https://doi.org/10.3390/bios8010019

A DNA-Based Assay for Digoxin Detection

Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark
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Received: 6 February 2018 / Revised: 27 February 2018 / Accepted: 1 March 2018 / Published: 6 March 2018
(This article belongs to the Special Issue FRET-Based Biosensors)
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Abstract

The most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography in combination with mass spectrometry. Few immuno-based assays are available for the detection of small-molecule drugs in blood. Here we report on a homogeneous assay that enables detection of the concentration of digoxin spiked into in a plasma sample. The assay is based on a shift in the equilibrium of a DNA strand displacement competition reaction, and can be performed in 30 min for concentrations above 10 nM. The equilibrium shift occurs upon binding of anti-digoxigenin antibody. As a model, the assay provides a potential alternative to current small-molecule detection methods used for therapeutic drug monitoring. View Full-Text
Keywords: digoxin detection; homogeneous assay; DNA nanotechnology; DNA strand displacement competition reaction; detection of small molecules; detection in plasma digoxin detection; homogeneous assay; DNA nanotechnology; DNA strand displacement competition reaction; detection of small molecules; detection in plasma
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kjelstrup, M.V.; Nielsen, L.D.F.; Hansen-Bruhn, M.; Gothelf, K.V. A DNA-Based Assay for Digoxin Detection. Biosensors 2018, 8, 19.

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