A DNA-Based Assay for Digoxin Detection
AbstractThe most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography in combination with mass spectrometry. Few immuno-based assays are available for the detection of small-molecule drugs in blood. Here we report on a homogeneous assay that enables detection of the concentration of digoxin spiked into in a plasma sample. The assay is based on a shift in the equilibrium of a DNA strand displacement competition reaction, and can be performed in 30 min for concentrations above 10 nM. The equilibrium shift occurs upon binding of anti-digoxigenin antibody. As a model, the assay provides a potential alternative to current small-molecule detection methods used for therapeutic drug monitoring. View Full-Text
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Kjelstrup, M.V.; Nielsen, L.D.F.; Hansen-Bruhn, M.; Gothelf, K.V. A DNA-Based Assay for Digoxin Detection. Biosensors 2018, 8, 19.
Kjelstrup MV, Nielsen LDF, Hansen-Bruhn M, Gothelf KV. A DNA-Based Assay for Digoxin Detection. Biosensors. 2018; 8(1):19.Chicago/Turabian Style
Kjelstrup, Michael V.; Nielsen, Line D.F.; Hansen-Bruhn, Malthe; Gothelf, Kurt V. 2018. "A DNA-Based Assay for Digoxin Detection." Biosensors 8, no. 1: 19.
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