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Biosensors 2018, 8(1), 20; https://doi.org/10.3390/bios8010020

Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection

1
Institute of Experimental and Clinical Pharmacology and Toxicology, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany
2
The Department of Pharmaceutical Biosciences, Uppsala University, 75124 Uppsala, Sweden
3
The Department of Physiology and Pharmacology, Karolinska Institutet, 17177 Stockholm, Sweden
4
CenMeT, University of Kentucky, Lexington, 40506 KY, USA
5
Quanteon LLC, Nicholasville, 40356 KY, USA
*
Author to whom correspondence should be addressed.
Received: 1 February 2018 / Revised: 15 February 2018 / Accepted: 2 March 2018 / Published: 6 March 2018
(This article belongs to the Special Issue Implantable Biosensors for in vivo Detection and Measurement)
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Abstract

D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO). We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 ± 0.83 pA/µM to D-serine, a limit of detection (LOD) of 0.17 ± 0.01 µM, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean ± SEM; n = 12) that can be used for freely moving studies. View Full-Text
Keywords: D-serine; biosensor; microelectrode array; amperometry; self-referencing D-serine; biosensor; microelectrode array; amperometry; self-referencing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Campos-Beltrán, D.; Konradsson-Geuken, Å.; Quintero, J.E.; Marshall, L. Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection. Biosensors 2018, 8, 20.

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