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Biosensors 2017, 7(4), 58; doi:10.3390/bios7040058

An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay

1
School of Aerospace Engineering, Sapienza University of Rome, via Salaria n. 851/881, 00138 Rome, Italy
2
Department of Chemistry, Sapienza University of Rome, p.le Aldo Moro n.5, 00185 Rome, Italy
3
Mesoscale Chemical Systems, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
4
NanoLab cleanroom, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands
5
Department of Environmental Biology, Sapienza University of Rome, p.le Aldo Moro n.5, 00185 Rome Italy
6
Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Via Eudossiana, 18, 00184 Rome, Italy
*
Author to whom correspondence should be addressed.
Received: 27 October 2017 / Revised: 1 December 2017 / Accepted: 2 December 2017 / Published: 5 December 2017
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Abstract

A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 μM by using luminol and 4-iodophenol as enhancer agent. View Full-Text
Keywords: microfluidic channel; surface functionalization; amorphous silicon photosensors; chemiluminescent based assay; lab-on-chip microfluidic channel; surface functionalization; amorphous silicon photosensors; chemiluminescent based assay; lab-on-chip
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Costantini, F.; Tiggelaar, R.M.; Salvio, R.; Nardecchia, M.; Schlautmann, S.; Manetti, C.; Gardeniers, H.J.G.E.; de Cesare, G.; Caputo, D.; Nascetti, A. An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay. Biosensors 2017, 7, 58.

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