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Biosensors 2015, 5(2), 308-318; doi:10.3390/bios5020308

Multi-Pixel Photon Counters for Optofluidic Characterization of Particles and Microalgae

Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA
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Author to whom correspondence should be addressed.
Academic Editor: Jeff D. Newman
Received: 30 March 2015 / Accepted: 9 June 2015 / Published: 12 June 2015
(This article belongs to the Special Issue Biosensors in Environmental Studies)
View Full-Text   |   Download PDF [571 KB, uploaded 12 June 2015]   |  

Abstract

We have developed an optofluidic biosensor to study microscale particles and different species of microalgae. The system is comprised of a microchannel with a set of chevron-shaped grooves. The chevrons allows for hydrodynamic focusing of the core stream in the center using a sheath fluid. The device is equipped with a new generation of highly sensitive photodetectors, multi-pixel photon counter (MPPC), with high gain values and an extremely small footprint. Two different sizes of high intensity fluorescent microspheres and three different species of algae (Chlamydomonas reinhardtii strain 21 gr, Chlamydomonas suppressor, and Chlorella sorokiniana) were studied. The forward scattering emissions generated by samples passing through the interrogation region were carried through a multimode fiber, located in 135 degree with respect to the excitation fiber, and detected by a MPPC. The signal outputs obtained from each sample were collected using a data acquisition system and utilized for further statistical analysis. Larger particles or cells demonstrated larger peak height and width, and consequently larger peak area. The average signal output (integral of the peak) for Chlamydomonas reinhardtii strain 21 gr, Chlamydomonas suppressor, and Chlorella sorokiniana falls between the values found for the 3.2 and 10.2 μm beads. Different types of algae were also successfully characterized. View Full-Text
Keywords: optofluidics; microfluidics; multi-pixel photon counter optofluidics; microfluidics; multi-pixel photon counter
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Asrar, P.; Sucur, M.; Hashemi, N. Multi-Pixel Photon Counters for Optofluidic Characterization of Particles and Microalgae. Biosensors 2015, 5, 308-318.

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