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Biosensors 2015, 5(2), 319-336; doi:10.3390/bios5020319

Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing

1
Environmental Biogeochemistry and Ecotoxicology, Institute F.-A. Forel, Earth and Environmental Science, Faculty of Sciences, University of Geneva, 10 route de Suisse, Versoix CH-1290, Switzerland
2
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Christophe A. Marquette
Received: 13 April 2015 / Revised: 31 May 2015 / Accepted: 5 June 2015 / Published: 15 June 2015
(This article belongs to the Special Issue Cell and Organ on Chip: Challenges and Advances)
View Full-Text   |   Download PDF [1144 KB, uploaded 15 June 2015]   |  

Abstract

An alternative current (AC) dielectrophoretic lab-on-chip setup was evaluated as a rapid tool of capture and assembly of microalga Chlamydomonas reinhardtii in two-dimensional (2D) close-packed arrays. An electric field of 100 V·cm−1, 100 Hz applied for 30 min was found optimal to collect and assemble the algae into single-layer structures of closely packed cells without inducing cellular oxidative stress. Combined with oxidative stress specific staining and fluorescence microscopy detection, the capability of using the 2D whole-cell assembly on-chip to follow the reactive oxygen species (ROS) production and oxidative stress during short-term exposure to several environmental contaminants, including mercury, methylmercury, copper, copper oxide nanoparticles (CuO-NPs), and diuron was explored. The results showed significant increase of the cellular ROS when C. reinhardtii was exposed to high concentrations of methylmercury, CuO-NPs, and 10−5 M Cu. Overall, this study demonstrates the potential of combining AC-dielectrophoretically assembled two-dimensional algal structures with cell metabolic analysis using fluorescence staining, as a rapid analytical tool for probing the effect of contaminants in highly impacted environment. View Full-Text
Keywords: whole cell assembly; dielectrophoresis; fluorescence; contamination; reactive oxygen species; Chlamydomonas reinhardtii whole cell assembly; dielectrophoresis; fluorescence; contamination; reactive oxygen species; Chlamydomonas reinhardtii
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Siebman, C.; Velev, O.D.; Slaveykova, V.I. Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing. Biosensors 2015, 5, 319-336.

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