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Biosensors 2015, 5(2), 223-240; doi:10.3390/bios5020223

Total Internal Reflection Fluorescence Quantification of Receptor Pharmacology

Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA
Academic Editor: A. Sheila Holmes-Smith
Received: 22 March 2015 / Revised: 15 April 2015 / Accepted: 17 April 2015 / Published: 27 April 2015
(This article belongs to the Special Issue Fluorescence Based Sensing Technologies)
View Full-Text   |   Download PDF [694 KB, uploaded 27 April 2015]   |  

Abstract

Total internal reflection fluorescence (TIRF) microscopy has been widely used as a single molecule imaging technique to study various fundamental aspects of cell biology, owing to its ability to selectively excite a very thin fluorescent volume immediately above the substrate on which the cells are grown. However, TIRF microscopy has found little use in high content screening due to its complexity in instrumental setup and experimental procedures. Inspired by the recent demonstration of label-free evanescent wave biosensors for cell phenotypic profiling and drug screening with high throughput, we had hypothesized and demonstrated that TIRF imaging is also amenable to receptor pharmacology profiling. This paper reviews key considerations and recent applications of TIRF imaging for pharmacology profiling. View Full-Text
Keywords: evanescent wave; receptor pharmacology; resonant waveguide grating; single molecule analysis; surface plasmon resonance; total internal reflection fluorescence evanescent wave; receptor pharmacology; resonant waveguide grating; single molecule analysis; surface plasmon resonance; total internal reflection fluorescence
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fang, Y. Total Internal Reflection Fluorescence Quantification of Receptor Pharmacology. Biosensors 2015, 5, 223-240.

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