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Biosensors 2015, 5(2), 199-222; doi:10.3390/bios5020199

Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time

1
Department of Pharmacology, University of Auckland, Auckland 1023, New Zealand
2
Centre for Brain Research, University of Auckland, Auckland 1023, New Zealand
3
School of Medical Sciences, University of Auckland, Auckland 1023, New Zealand
4
Department of Engineering Science, University of Auckland, Auckland 1010, New Zealand
5
Department of Anatomy with Radiology, University of Auckland, Auckland 1023, New Zealand
6
School of Biological Sciences, University of Auckland, Auckland 1010, New Zealand
*
Author to whom correspondence should be addressed.
Academic Editor: Jeff D. Newman
Received: 20 February 2015 / Revised: 27 March 2015 / Accepted: 2 April 2015 / Published: 16 April 2015
View Full-Text   |   Download PDF [893 KB, uploaded 16 April 2015]   |  

Abstract

The xCELLigence technology is a real-time cellular biosensor, which measures the net adhesion of cells to high-density gold electrode arrays printed on custom-designed E-plates. The strength of cellular adhesion is influenced by a myriad of factors that include cell type, cell viability, growth, migration, spreading and proliferation. We therefore hypothesised that xCELLigence biosensor technology would provide a valuable platform for the measurement of drug responses in a multitude of different experimental, clinical or pharmacological contexts. In this manuscript, we demonstrate how xCELLigence technology has been invaluable in the identification of (1) not only if cells respond to a particular drug, but (2) the window of drug responsiveness. The latter aspect is often left to educated guess work in classical end-point assays, whereas biosensor technology reveals the temporal profile of the response in real time, which enables both acute responses and longer term responses to be profiled within the same assay. In our experience, the xCELLigence biosensor technology is suitable for highly targeted drug assessment and also low to medium throughput drug screening, which produces high content temporal data in real time. View Full-Text
Keywords: xCELLigence; adhesion; cytotoxicity; cell death; drugs; responsiveness; Cell Index xCELLigence; adhesion; cytotoxicity; cell death; drugs; responsiveness; Cell Index
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kho, D.; MacDonald, C.; Johnson, R.; Unsworth, C.P.; O'Carroll, S.J.; Mez, E.D.; Angel, C.E.; Graham, E.S. Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time. Biosensors 2015, 5, 199-222.

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