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Biosensors 2015, 5(2), 337-363; doi:10.3390/bios5020337

Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances

1
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan
2
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: A. Sheila Holmes-Smith
Received: 22 April 2015 / Revised: 8 June 2015 / Accepted: 9 June 2015 / Published: 18 June 2015
(This article belongs to the Special Issue Fluorescence Based Sensing Technologies)

Abstract

This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques. View Full-Text
Keywords: molecular probes; sensors; metal ions; proteins; DNA molecular probes; sensors; metal ions; proteins; DNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Suzuki, Y.; Yokoyama, K. Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances. Biosensors 2015, 5, 337-363.

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