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Micropatterning of 3D Microenvironments for Living Biosensor Applications
AbstractMicro-scale printing and patterning of living cells has multiple applications including tissue engineering, cell signaling assays, and the fabrication of cell-based biosensors. In this work, a molecular printing instrument, the Bioforce Nano eNabler, was modified to enable micron-scale “quill-pen” based printing of mammalian cells in a 3D hyaluronan/gelatin based hydrogel. Specifically, photo-initiated “thiol-ene” click chemistry was used to couple the thiol groups of thiolated hyaluronan/thiolated gelatin to the alkene groups of 4-arm polyethylene glycol (PEG)-norbornene molecules. Rapid photopolymerization enabled direct printing and controlled curing of living cells within the hydrogel matrix. The resulting hydrogels were biocompatible with human adipose-derived stem cells, NIH-3T3 cells, and mouse embryonic stem cells. The utility of this printing approach was also explored for cell-based biosensors. Micro-printed cells expressing a redox sensitive variant of the green fluorescent protein (roGFP-R12) showed a measurable fluorescent response to addition of oxidizing and then reducing agents. This work represents a novel approach to micron-scale cell patterning, and its potential for living, cell-based biosensors.
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Hynes, W.F.; Doty, N.J.; Zarembinski, T.I.; Schwartz, M.P.; Toepke, M.W.; Murphy, W.L.; Atzet, S.K.; Clark, R.; Melendez, J.A.; Cady, N.C. Micropatterning of 3D Microenvironments for Living Biosensor Applications. Biosensors 2014, 4, 28-44.View more citation formats
Hynes WF, Doty NJ, Zarembinski TI, Schwartz MP, Toepke MW, Murphy WL, Atzet SK, Clark R, Melendez JA, Cady NC. Micropatterning of 3D Microenvironments for Living Biosensor Applications. Biosensors. 2014; 4(1):28-44.Chicago/Turabian Style
Hynes, William F.; Doty, Nate J.; Zarembinski, Thomas I.; Schwartz, Michael P.; Toepke, Michael W.; Murphy, William L.; Atzet, Sarah K.; Clark, Ryan; Melendez, J. A.; Cady, Nathaniel C. 2014. "Micropatterning of 3D Microenvironments for Living Biosensor Applications." Biosensors 4, no. 1: 28-44.