Biosensors 2014, 4(1), 18-27; doi:10.3390/bios4010018
Article

Microfluidic Platform for the Elastic Characterization of Mouse Submandibular Glands by Atomic Force Microscopy

1email, 2,†email, 2,†email and 1,* email
Received: 24 December 2013; in revised form: 4 February 2014 / Accepted: 17 February 2014 / Published: 27 February 2014
(This article belongs to the Special Issue Sensors and Analytics for Cell Biology and Tissue Engineering)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The ability to characterize the microscale mechanical properties of biological materials has the potential for great utility in the field of tissue engineering. The development and morphogenesis of mammalian tissues are known to be guided in part by mechanical stimuli received from the local environment, and tissues frequently develop to match the physical characteristics (i.e., elasticity) of their environment. Quantification of these material properties at the microscale may provide valuable information to guide researchers. Presented here is a microfluidic platform for the non-destructive ex vivo microscale mechanical characterization of mammalian tissue samples by atomic force microscopy (AFM). The device was designed to physically hold a tissue sample in a dynamically controllable fluid environment while allowing access by an AFM probe operating in force spectroscopy mode to perform mechanical testing. Results of measurements performed on mouse submandibular gland samples demonstrate the ability of the analysis platform to quantify sample elasticity at the microscale, and observe chemically-induced changes in elasticity.
Keywords: microfluidics; elasticity; tissue engineering; submandibular gland; AFM
PDF Full-text Download PDF Full-Text [318 KB, uploaded 27 February 2014 08:35 CET]

Export to BibTeX |
EndNote


MDPI and ACS Style

Mosier, A.P.; Peters, S.B.; Larsen, M.; Cady, N.C. Microfluidic Platform for the Elastic Characterization of Mouse Submandibular Glands by Atomic Force Microscopy. Biosensors 2014, 4, 18-27.

AMA Style

Mosier AP, Peters SB, Larsen M, Cady NC. Microfluidic Platform for the Elastic Characterization of Mouse Submandibular Glands by Atomic Force Microscopy. Biosensors. 2014; 4(1):18-27.

Chicago/Turabian Style

Mosier, Aaron P.; Peters, Sarah B.; Larsen, Melinda; Cady, Nathaniel C. 2014. "Microfluidic Platform for the Elastic Characterization of Mouse Submandibular Glands by Atomic Force Microscopy." Biosensors 4, no. 1: 18-27.

Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert