Next Article in Journal
Previous Article in Journal
Previous Article in Special Issue
Biosensors 2013, 3(1), 132-156; doi:10.3390/bios3010132
Article

Evaluation of Impedance-Based Label-Free Technology as a Tool for Pharmacology and Toxicology Investigations

* ,
,
,
 and
Received: 1 January 2013; in revised form: 15 February 2013 / Accepted: 27 February 2013 / Published: 15 March 2013
(This article belongs to the Special Issue Multiplex and Multiparametric Sensing Devices)
View Full-Text   |   Download PDF [987 KB, uploaded 15 March 2013]
Abstract: The use of label-free technologies based on electrical impedance is becoming more and more popular in drug discovery. Indeed, such a methodology allows the continuous monitoring of diverse cellular processes, including proliferation, migration, cytotoxicity and receptor-mediated signaling. The objective of the present study was to further assess the usefulness of the real-time cell analyzer (RTCA) and, in particular, the xCELLigence platform, in the context of early drug development for pharmacology and toxicology investigations. In the present manuscript, four cellular models were exposed to 50 compounds to compare the cell index generated by RTCA and cell viability measured with a traditional viability assay. The data revealed an acceptable correlation (ca. 80%) for both cell lines (i.e., HepG2 and HepaRG), but a lack of correlation (ca. 55%) for the primary human and rat hepatocytes. In addition, specific RTCA profiles (signatures) were generated when HepG2 and HepaRG cells were exposed to calcium modulators, antimitotics, DNA damaging and nuclear receptor agents, with a percentage of prediction close to 80% for both cellular models. In a subsequent experiment, HepG2 cells were exposed to 81 proprietary UCB compounds known to be genotoxic or not. Based on the DNA damaging signatures, the RTCA technology allowed the detection of ca. 50% of the genotoxic compounds (n = 29) and nearly 100% of the non-genotoxic compounds (n = 52). Overall, despite some limitations, the xCELLigence platform is a powerful and reliable tool that can be used in drug discovery for toxicity and pharmacology studies.
Keywords: real-time cell analyzer (RTCA); impedance; drug discovery; investigative pharmacology; predictive toxicology; screening real-time cell analyzer (RTCA); impedance; drug discovery; investigative pharmacology; predictive toxicology; screening
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Atienzar, F.A.; Gerets, H.; Tilmant, K.; Toussaint, G.; Dhalluin, S. Evaluation of Impedance-Based Label-Free Technology as a Tool for Pharmacology and Toxicology Investigations. Biosensors 2013, 3, 132-156.

AMA Style

Atienzar FA, Gerets H, Tilmant K, Toussaint G, Dhalluin S. Evaluation of Impedance-Based Label-Free Technology as a Tool for Pharmacology and Toxicology Investigations. Biosensors. 2013; 3(1):132-156.

Chicago/Turabian Style

Atienzar, Franck A.; Gerets, Helga; Tilmant, Karen; Toussaint, Gaëlle; Dhalluin, Stéphane. 2013. "Evaluation of Impedance-Based Label-Free Technology as a Tool for Pharmacology and Toxicology Investigations." Biosensors 3, no. 1: 132-156.

Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert