Biosensors 2013, 3(1), 157-170; doi:10.3390/bios3010157
Article

Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging

1,†,* email, 2email, 3,4email and 1,4email
Received: 23 January 2013; in revised form: 23 February 2013 / Accepted: 6 March 2013 / Published: 19 March 2013
(This article belongs to the Special Issue Immunosensors 2012)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor—estrone—was attached and detected in deionized water with a diverging beam SPR imaging sensor.
Keywords: optical immunosensor; immobilization; estrone; endocrine disruptor; SPR imaging
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MDPI and ACS Style

Karabchevsky, A.; Tsapovsky, L.; Marks, R.S.; Abdulhalim, I. Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging. Biosensors 2013, 3, 157-170.

AMA Style

Karabchevsky A, Tsapovsky L, Marks RS, Abdulhalim I. Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging. Biosensors. 2013; 3(1):157-170.

Chicago/Turabian Style

Karabchevsky, Alina; Tsapovsky, Lev; Marks, Robert S.; Abdulhalim, Ibrahim. 2013. "Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging." Biosensors 3, no. 1: 157-170.

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