Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using Hydra Proteins and Peptides during Zebrafish Embryogenesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of N-Ag-PVP, HP, and Hym176
2.2. Physicochemical Properties of N-Ag-PVP and N-Ag-PVP+HP
2.3. Experimental Animals
2.4. Exposure Conditions
2.5. Microarray Analysis
2.6. qRT-PCR Analysis
2.7. Statistical Analysis
3. Results and Discussion
3.1. Properties of N-Ag-PVP and HP
3.2. Effects of HP and Hym176 on N-Ag-PVP-Exposed Zebrafish Embryos
3.3. Microarray and qRT-PCR Analyses
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | Gene Symbol | Function | Accession No. | Primer Sequences (5′ → 3′) |
---|---|---|---|---|
claudin a | cldna | structural molecule activity | NM _131762 | (F) TGTGGCAAGTCACTGCTTTT (R) CACGCAACTCATCCAAATTC |
cell division cycle 42, like | cdc42l | GTP binding, GTPase activity, nucleotide binding, protein kinase binding | NM _199865 | (F) GGCAGGAAGACTACGACAGA (R) GGAGGAAGGAGAAACAACTGA |
insulin-like growth factor 1 | igf1 | Growth factor activity, hormone activity, insulin-like growth factor receptor binding | NM _131825 | (F) GTGGACGAATGCTGCTTTCA (R) CTGTCTTCACAGGCGCACAA |
zgc:55558 | zgc:55558 | GTP binding, GTPase activity, nucleotide binding, | NM _200258 | (F) TTTTTACACCCCCATCCTTT (R) GGTCTCGTGTGCACAGACAT |
mitogen-activated protein kinase 10 | mapk10 | ATP binding, JUN kinase activity, MAP kinase activity, kinase activity, nucleotide binding, protein kinase activity, protein serine/threonine kinase activity, transferase activity | NM _001037701 | (F) TCGAGGAGAGAACAAAGAATGG (R) AGGCTCTCGCTGCTGTTCAC |
mitogen-activated protein kinase 11 | mapk11 | ATP binding, MAP kinase activity, kinase activity, nucleotide binding, protein kinase activity, protein serine/threonine kinase activity, transferase activity | NM _001002095 | (F) CAGTACTGCCCTCTCCTTCTT (R) ATCGTCTCGTCTGGCTGAAC |
tumor protein p53 | tp53 | DNA binding, DNA-binding transcription factor activity, metal ion binding, promoter-specific chromatin binding, protein binding, sequence-specific DNA binding, transcription regulatory region DNA binding, | NM _001271820 | (F) TGCGATACATGTGATCCATT (R) CAGTGTCCAGCAACAAAGGT |
actin, beta 2 | actb2 | ATP binding, nucleotide binding | NM _181601 | (F) GACTCAAACTGCGCAGAGAA (R) AGTCAAGCGCCAAAAATAAC |
N-Ag-PVP | N-Ag-PVP+HP | |
---|---|---|
Zeta potential (mV) | −22.84 ± 0.16 | −5.54 ± 0.80 |
Diameter (nm) | 285.50 ± 16.40 | 534.40 ± 122.05 |
Total Genes | Up-Regulated | Down-Regulated | ||
---|---|---|---|---|
N-Ag-PVP | vs. Control | 957 (6.61%) | 377 | 580 |
HP | vs. Control | 2686 (18.56%) | 1066 | 1620 |
N-Ag-PVP+HP | vs. Control | 928 (6.41%) | 379 | 549 |
Hym176 | vs. Control | 1229 (8.49%) | 545 | 684 |
N-Ag-PVP+Hym176 | vs. Control | 1284 (8.87%) | 632 | 652 |
N-Ag-PVP+HP | vs. HP | 2879 (19.89%) | 1652 | 1227 |
N-Ag-PVP+Hym176 | vs. Hym176 | 164 (1.13%) | 112 | 52 |
N-Ag-PVP+Hym176 | vs. N-Ag-PVP+HP | 353 (2.44%) | 202 | 151 |
N-Ag-PVP+HP | vs. N-Ag-PVP | 110 (0.76%) | 49 | 61 |
N-Ag-PVP+Hym176 | vs. N-Ag-PVP | 552 (3.81%) | 332 | 220 |
Gene Description | Gene Symbol | Regulation Profile and Ratio(Fold change) | ||||
---|---|---|---|---|---|---|
N-Ag-PVP /Control | HP /Control | N-Ag-PVP+HP /Control | Hym176 /Control | N-Ag-PVP +Hym176 /Control | ||
Heart morphogenesis (GO:0003007) | ||||||
LIM domain 7b | lmo7b | 2.708 | 0.743 | 1.415 | 2.264 | 1.425 |
fibronectin 1b | fn1b | 2.179 | 1.643 | 1.723 | 3.874 | 5.859 |
myosin binding protein C, cardiac | mybpc3 | 0.357 | 1.706 | 0.569 | 0.493 | 0.959 |
Fin morphogenesis (GO:0033334) | ||||||
Sec23 homolog A, COPII coat complex component | sec23a | 0.448 | 1.369 | 0.709 | 0.588 | 0.832 |
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a | plod1a | 0.441 | 0.869 | 0.611 | 0.462 | 0.665 |
Embryonic organ morphogenesis (GO:0048562) | ||||||
LIM domain 7b | lmo7b | 2.708 | 0.743 | 1.415 | 2.264 | 1.425 |
lumican | lum | 0.491 | 1.359 | 0.620 | 0.701 | 0.625 |
Sec23 homolog A, COPII coat complex component | sec23a | 0.448 | 1.369 | 0.709 | 0.588 | 0.832 |
Eye morphogenesis (GO:0048592) | ||||||
intraflagellar transport 80 homolog (Chlamydomonas) | ift80 | 2.179 | 0.722 | 1.356 | 1.416 | 1.203 |
tubulin, gamma complex associated protein 4 | tubgcp4 | 2.017 | 0.698 | 1.563 | 1.177 | 0.896 |
lumican | lum | 0.491 | 1.359 | 0.620 | 0.701 | 0.625 |
adiponectin receptor 1b | adipor1b | 0.444 | 0.924 | 0.620 | 0.271 | 0.371 |
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Kim, S.S.; Lee, J.A.; Yeo, M.-K. Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using Hydra Proteins and Peptides during Zebrafish Embryogenesis. Nanomaterials 2019, 9, 1210. https://doi.org/10.3390/nano9091210
Kim SS, Lee JA, Yeo M-K. Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using Hydra Proteins and Peptides during Zebrafish Embryogenesis. Nanomaterials. 2019; 9(9):1210. https://doi.org/10.3390/nano9091210
Chicago/Turabian StyleKim, Soon Seok, Jin Ah Lee, and Min-Kyeong Yeo. 2019. "Reduction in Toxicity of Nano-Ag-Polyvinyl-pyrrolidone Using Hydra Proteins and Peptides during Zebrafish Embryogenesis" Nanomaterials 9, no. 9: 1210. https://doi.org/10.3390/nano9091210