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Nanomaterials 2018, 8(5), 290; https://doi.org/10.3390/nano8050290

Biofunctionalized Lysophosphatidic Acid/Silk Fibroin Film for Cornea Endothelial Cell Regeneration

1
Department of BIN Convergence Technology, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Korea
2
Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Chonbuk National University, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Korea
3
3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark—Parque de Ciência e Tecnologia, Zona Industrial de Gandra, 4805-017 Barco, Guimarães, Portugal
4
ICVS/3B’s—PT Government Associated Laboratory, Braga/Guimarães, Portugal; The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, 4805-017 Barco, Guimarães, Portugal
*
Author to whom correspondence should be addressed.
Received: 28 March 2018 / Revised: 25 April 2018 / Accepted: 25 April 2018 / Published: 30 April 2018
(This article belongs to the Special Issue Tissue Engineering and Regenerative Nanomedicine)
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Abstract

Cornea endothelial cells (CEnCs) tissue engineering is a great challenge to repair diseased or damaged CEnCs and require an appropriate biomaterial to support cell proliferation and differentiation. Biomaterials for CEnCs tissue engineering require biocompatibility, tunable biodegradability, transparency, and suitable mechanical properties. Silk fibroin-based film (SF) is known to meet these factors, but construction of functionalized graft for bioengineering of cornea is still a challenge. Herein, lysophosphatidic acid (LPA) is used to maintain and increase the specific function of CEnCs. The LPA and SF composite film (LPA/SF) was fabricated in this study. Mechanical properties and in vitro studies were performed using a rabbit model to demonstrate the characters of LPA/SF. ATR-FTIR was characterized to identify chemical composition of the films. The morphological and physical properties were performed by SEM, AFM, transparency, and contact angle. Initial cell density and MTT were performed for adhesion and cell viability in the SF and LPA/SF film. Reverse transcription polymerase chain reactions (RT-PCR) and immunofluorescence were performed to examine gene and protein expression. The results showed that films were designed appropriately for CEnCs delivery. Compared to pristine SF, LPA/SF showed higher biocompatibility, cell viability, and expression of CEnCs specific genes and proteins. These indicate that LPA/SF, a new biomaterial, offers potential benefits for CEnCs tissue engineering for regeneration. View Full-Text
Keywords: cornea endothelial cells; tissue engineering; regeneration; silk fibroin; lysophosphatidic acid cornea endothelial cells; tissue engineering; regeneration; silk fibroin; lysophosphatidic acid
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Choi, J.H.; Jeon, H.; Song, J.E.; Oliveira, J.M.; Reis, R.L.; Khang, G. Biofunctionalized Lysophosphatidic Acid/Silk Fibroin Film for Cornea Endothelial Cell Regeneration. Nanomaterials 2018, 8, 290.

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