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Nanomaterials 2017, 7(7), 153; doi:10.3390/nano7070153

The Electrosome: A Surface-Displayed Enzymatic Cascade in a Biofuel Cell’s Anode and a High-Density Surface-Displayed Biocathodic Enzyme

1
Department of Life Sciences and the Ilse Katz Institute for Nanoscale Science and Technology, P.O. Box 653, 8410501 Beer-Sheva, Israel
2
Department of Biomolecular Sciences, Weizmann Institute of Science, 234 Herzl St., P.O. Box 26, 7610001 Rehovot, Israel
3
Department of Chemical Research Support, Weizmann Institute of Science, 234 Herzl St., P.O. Box 26, 7610001 Rehovot, Israel
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas Nann
Received: 1 May 2017 / Revised: 12 June 2017 / Accepted: 20 June 2017 / Published: 23 June 2017
(This article belongs to the Special Issue Nanostructured Biofuel Cells)
View Full-Text   |   Download PDF [2226 KB, uploaded 23 June 2017]   |  

Abstract

The limitation of surface-display systems in biofuel cells to a single redox enzyme is a major drawback of hybrid biofuel cells, resulting in a low copy-number of enzymes per yeast cell and a limitation in displaying enzymatic cascades. Here we present the electrosome, a novel surface-display system based on the specific interaction between the cellulosomal scaffoldin protein and a cascade of redox enzymes that allows multiple electron-release by fuel oxidation. The electrosome is composed of two compartments: (i) a hybrid anode, which consists of dockerin-containing enzymes attached specifically to cohesin sites in the scaffoldin to assemble an ethanol oxidation cascade, and (ii) a hybrid cathode, which consists of a dockerin-containing oxygen-reducing enzyme attached in multiple copies to the cohesin-bearing scaffoldin. Each of the two compartments was designed, displayed, and tested separately. The new hybrid cell compartments displayed enhanced performance over traditional biofuel cells; in the anode, the cascade of ethanol oxidation demonstrated higher performance than a cell with just a single enzyme. In the cathode, a higher copy number per yeast cell of the oxygen-reducing enzyme copper oxidase has reduced the effect of competitive inhibition resulting from yeast oxygen consumption. This work paves the way for the assembly of more complex cascades using different enzymes and larger scaffoldins to further improve the performance of hybrid cells. View Full-Text
Keywords: hybrid biofuel cells; enzymatic cascades; scaffoldin; cohesin; dockerin; redox enzymes; cellulosome hybrid biofuel cells; enzymatic cascades; scaffoldin; cohesin; dockerin; redox enzymes; cellulosome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Szczupak, A.; Aizik, D.; Moraïs, S.; Vazana, Y.; Barak, Y.; Bayer, E.A.; Alfonta, L. The Electrosome: A Surface-Displayed Enzymatic Cascade in a Biofuel Cell’s Anode and a High-Density Surface-Displayed Biocathodic Enzyme. Nanomaterials 2017, 7, 153.

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