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Nanomaterials 2017, 7(5), 110; doi:10.3390/nano7050110

Magnetic Cationic Amylose Nanoparticles Used to Deliver Survivin-Small Interfering RNA for Gene Therapy of Hepatocellular Carcinoma In Vitro

1
Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China
2
Department of Ultrasound, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China
3
Department of Polymer and Materials Science, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China
4
School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China
5
Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Guangdong Provincial Key Laboratory for High Performance Polymer-Based Composites, Key Laboratory of Designed Synthesis and Application of Polymer Material, Sun Yat-Sen University, Guangzhou 510275, China
These authors contribute equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Manh-Huong Phan
Received: 1 April 2017 / Revised: 7 May 2017 / Accepted: 9 May 2017 / Published: 11 May 2017
(This article belongs to the Special Issue Functional Magnetic Nanoparticles in Nanomedicine)
View Full-Text   |   Download PDF [5461 KB, uploaded 11 May 2017]   |  

Abstract

Amylose is a promising nanocarrier for gene delivery in terms of its good biocompatibility and high transfection efficiency. Small interfering RNA against survivin (survivin-siRNA) can cause tumor apoptosis by silencing a hepatocellular carcinoma (HCC)-specific gene at the messenger RNA level. In this study, we developed a new class of folate-functionalized, superparamagnetic iron oxide (SPIO)-loaded cationic amylose nanoparticles to deliver survivin-siRNA to HCC cells. The cellular uptake of nanocomplexes, cytotoxicity, cell apoptosis, and gene suppression mediated by siRNA-complexed nanoparticles were tested. The results demonstrated that folate-functionalized, SPIO-loaded cationic amylose nanoparticles can mediate a specific and safe cellular uptake of survivin-siRNA with high transfection efficiency, resulting in a robust survivin gene downregulation in HCC cells. The biocompatible complex of cationic amylose could be used as an efficient, rapid, and safe gene delivery vector. Upon SPIO loading, it holds a great promise as a theranostic carrier for gene therapy of HCC. View Full-Text
Keywords: amylose; small interfering RNA; magnetic resonance imaging; superparamagnetic iron oxide nanoparticles amylose; small interfering RNA; magnetic resonance imaging; superparamagnetic iron oxide nanoparticles
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wu, Z.; Xu, X.-L.; Zhang, J.-Z.; Mao, X.-H.; Xie, M.-W.; Cheng, Z.-L.; Lu, L.-J.; Duan, X.-H.; Zhang, L.-M.; Shen, J. Magnetic Cationic Amylose Nanoparticles Used to Deliver Survivin-Small Interfering RNA for Gene Therapy of Hepatocellular Carcinoma In Vitro. Nanomaterials 2017, 7, 110.

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