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Nanomaterials 2017, 7(5), 107; doi:10.3390/nano7050107

Superparamagnetic Iron Oxide Nanoparticles-Complexed Cationic Amylose for In Vivo Magnetic Resonance Imaging Tracking of Transplanted Stem Cells in Stroke

1
Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China
2
Department of Polymer and Materials Science, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, Guangdong, China
3
School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China
4
Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Guangdong Provincial Key Laboratory for High Performance Polymer-Based Composites, Key Laboratory of Designed Synthesis and Application of Polymer Material, Sun Yat-Sen University, Guangzhou 510275, China
These authors contribute equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Manh-Huong Phan
Received: 30 March 2017 / Revised: 27 April 2017 / Accepted: 8 May 2017 / Published: 10 May 2017
(This article belongs to the Special Issue Functional Magnetic Nanoparticles in Nanomedicine)
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Abstract

Cell-based therapy with mesenchymal stem cells (MSCs) is a promising strategy for acute ischemic stroke. In vivo tracking of therapeutic stem cells with magnetic resonance imaging (MRI) is imperative for better understanding cellular survival and migrational dynamics over time. In this study, we develop a novel biocompatible nanocomplex (ASP-SPIONs) based on cationic amylose, by introducing spermine and the image label, ultrasmall superparamagnetic iron oxide nanoparticles (SPIONs), to label MSCs. The capacity, efficiency, and cytotoxicity of the nanocomplex in transferring SPIONs into green fluorescence protein-modified MSCs were tested; and the performance of in vivo MRI tracking of the transplanted cells in acute ischemic stroke was determined. The results demonstrated that the new class of SPIONs-complexed nanoparticles based on biodegradable amylose can serve as a highly effective and safe carrier to transfer magnetic label into stem cells. A reliable tracking of transplanted stem cells in stroke was achieved by MRI up to 6 weeks, with the desirable therapeutic benefit of stem cells on stroke retained. With the advantages of a relatively low SPIONs concentration and a short labeling period, the biocompatible complex of cationic amylose with SPIONs is highly translatable for clinical application. It holds great promise in efficient, rapid, and safe labeling of stem cells for subsequent cellular MRI tracking in regenerative medicine. View Full-Text
Keywords: superparamagnetic iron oxide nanoparticles; magnetic resonance imaging; amylose; ischemic stroke; mesenchymal stem cells; green fluorescence protein; biodegradation superparamagnetic iron oxide nanoparticles; magnetic resonance imaging; amylose; ischemic stroke; mesenchymal stem cells; green fluorescence protein; biodegradation
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Lin, B.-L.; Zhang, J.-Z.; Lu, L.-J.; Mao, J.-J.; Cao, M.-H.; Mao, X.-H.; Zhang, F.; Duan, X.-H.; Zheng, C.-S.; Zhang, L.-M.; Shen, J. Superparamagnetic Iron Oxide Nanoparticles-Complexed Cationic Amylose for In Vivo Magnetic Resonance Imaging Tracking of Transplanted Stem Cells in Stroke. Nanomaterials 2017, 7, 107.

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