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Nanomaterials 2017, 7(5), 100; doi:10.3390/nano7050100

A Large-Area Nanoplasmonic Sensor Fabricated by Rapid Thermal Annealing Treatment for Label-Free and Multi-Point Immunoglobulin Sensing

1
Graduate Institute of Biomedical Electronic and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan
2
Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan
3
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Guozhen Liu
Received: 17 February 2017 / Revised: 25 April 2017 / Accepted: 28 April 2017 / Published: 2 May 2017
(This article belongs to the Special Issue Nanomaterials for Sensing Applications)
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Abstract

Immunoglobulins are important biomarkers to evaluate the immune status or development of infectious diseases. To provide timely clinical treatments, it is important to continuously monitor the level of multiple immunoglobulins. Localized surface plasmon resonance (LSPR)-based nanoplasmonic sensors have been demonstrated for multiplex immunoglobulins detection. However, the sensor fabrication process is usually slow and complicated, so it is not accessible for large-area and batch fabrication. Herein, we report a large-area (2 cm × 2 cm) nanofabrication method using physical vapor deposition followed by a rapid thermal annealing treatment. To optimize the sensor performance, we systematically characterized three fabrication conditions, including (1) the deposition thickness; (2) the maximum annealing temperature, and (3) the annealing time. The corresponding absorbance spectrum profile and surface morphology of the nanostructures were observed by a UV-VIS spectrometer and atomic force microscopy. We then tested the sensitivity of the sensor using a glucose solution at different concentrations. The results showed that the sensor with 10 nm gold deposition thickness under 5-min 900 °C rapid thermal annealing can achieve the highest sensitivity (189 nm RIU−1). Finally, we integrated this nanoplasmonic sensor with a microchannel and a motorized stage to perform a 10-spot immunoglobulin detection in 50 min. Based on its real-time, dynamic and multi-point analyte detection capability, the nanoplasmonic sensor has the potential to be applied in high-throughput or multiplex immunoassay analysis, which would be beneficial for disease diagnosis or biomedical research in a simple and cost-effective platform. View Full-Text
Keywords: nanoplasmonic biosensing; localized surface plasmon resonance (LSPR); rapid thermal annealing (RTA) Treatment; label-free immunoassay nanoplasmonic biosensing; localized surface plasmon resonance (LSPR); rapid thermal annealing (RTA) Treatment; label-free immunoassay
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lin, H.T.-H.; Yang, C.-K.; Lin, C.-C.; Wu, A.M.-H.; Wang, L.A.; Huang, N.-T. A Large-Area Nanoplasmonic Sensor Fabricated by Rapid Thermal Annealing Treatment for Label-Free and Multi-Point Immunoglobulin Sensing. Nanomaterials 2017, 7, 100.

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