Next Article in Journal
Carbon Nanotube (CNT) Honeycomb Cell Area-Dependent Optical Reflectance
Next Article in Special Issue
Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines
Previous Article in Journal
Investigation of the Mechanical Properties and Microstructure of Graphene Nanoplatelet-Cement Composite
Previous Article in Special Issue
Pregnancy Vaccination with Gold Glyco-Nanoparticles Carrying Listeria monocytogenes Peptides Protects against Listeriosis and Brain- and Cutaneous-Associated Morbidities
Article Menu

Export Article

Open AccessFeature PaperArticle
Nanomaterials 2016, 6(11), 201; doi:10.3390/nano6110201

Nanoparticle-Based Delivery of Anaplasma marginale Membrane Proteins; VirB9-1 and VirB10 Produced in the Pichia pastoris Expression System

1
Department of Agriculture and Fisheries, Agri-Science Queensland, Animal Science, St Lucia, QLD 4072, Australia
2
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia
3
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia
4
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Neil O’Brien-Simpson
Received: 12 August 2016 / Revised: 21 October 2016 / Accepted: 28 October 2016 / Published: 5 November 2016
(This article belongs to the Special Issue Nanoparticles in Immunology)
View Full-Text   |   Download PDF [3046 KB, uploaded 5 November 2016]   |  

Abstract

Bovine anaplasmosis or cattle-tick fever is a tick-borne haemolytic disease caused by the rickettsial haemoparasite Anaplasma marginale in tropical and subtropical areas of the world. While difficult to express, the proteins VirB9-1 and VirB10 are immunogenic components of the outer membrane type IV secretion system that have been identified as candidate antigens for vaccines targeting of A. marginale. Soluble VirB9-1 and VirB10 were successfully expressed using Pichia pastoris. When formulated with the self-adjuvanting silica vesicles, SV-100 (diameter: 50 nm, and pore entrance size: 6 nm), 200 µg of VirB9-1 and VirB10 were adsorbed per milligram of nanoparticle. The VirB9-1 and VirB10, SV-100 formulations were shown to induce higher antibody responses in mice compared to the QuilA formulations. Moreover, intracellular staining of selected cytokines demonstrated that both VirB9-1 and VirB10 formulations induced cell-mediated immune responses in mice. Importantly, the SV-100 VirB9-1 and VirB10 complexes were shown to specifically stimulate bovine T-cell linages derived from calves immunised with A. marginale outer membrane fractions, suggesting formulations will be useful for bovine immunisation and protection studies. Overall this study demonstrates the potential of self-adjuvanting silica vesicle formulations to address current deficiencies in vaccine delivery applications. View Full-Text
Keywords: Anaplasma marginale; VirB9-1; VirB10; Pichia pastoris; silica vesicles; vaccine adjuvants; immune responses Anaplasma marginale; VirB9-1; VirB10; Pichia pastoris; silica vesicles; vaccine adjuvants; immune responses
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhang, B.; Cavallaro, A.S.; Mody, K.T.; Zhang, J.; Deringer, J.R.; Brown, W.C.; Mahony, T.J.; Yu, C.; Mitter, N. Nanoparticle-Based Delivery of Anaplasma marginale Membrane Proteins; VirB9-1 and VirB10 Produced in the Pichia pastoris Expression System. Nanomaterials 2016, 6, 201.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top