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Nanomaterials 2017, 7(2), 28; doi:10.3390/nano7020028

Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies

1
nanoTherics Limited, Keele University Science Park, Keele, ST5 5NL, UK
2
Takeda Cambridge, 418 Cambridge Science Park Milton Rd, Milton, Cambridge, CB4 0PZ, UK
3
Biological and Environmental Sciences, School of Natural Sciences, University of Stirling, Stirling, FK9 4LA, UK
4
J. Crayton Pruitt Family Department of Biomedical Engineering, Department of Material Science and Engineering, and Institute for Cell Engineering and Regenerative Medicine—ICERM, University of Florida, P.O. Box 116131, Gainesville, Florida, FL 32611, USA
Current Address: Miltenyi Biotec, Almac House, Church Lane, Bisley, Surrey, GU24 9DR, UK
Current Address: Alzheimer’s Research UK Oxford Drug Discovery Institute, University of Oxford, Oxford, OX3 7FZ, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Manh-Huong Phan
Received: 28 November 2016 / Revised: 20 January 2017 / Accepted: 23 January 2017 / Published: 29 January 2017
(This article belongs to the Special Issue Functional Magnetic Nanoparticles in Nanomedicine)
View Full-Text   |   Download PDF [1192 KB, uploaded 6 February 2017]   |  

Abstract

To develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. However, as cultured primary neurons are sensitive to temperature change, stress, and shifts in pH, these factors make biomolecule delivery difficult, particularly non-viral delivery. Herein we used oscillating nanomagnetic gene transfection to successfully transfect SH-SY5Y cells as well as primary hippocampal and cortical neurons on different days in vitro. This novel technique has been used to effectively deliver genetic material into various cell types, resulting in high transfection efficiency and viability. From these observations and other related studies, we suggest that oscillating nanomagnetic gene transfection is an effective method for gene delivery into hard-to-transfect neuronal cell types. View Full-Text
Keywords: magnetic nanoparticles; transfection; SH-SY5Y; hippocampal neurons; cortical neurons; non-viral gene delivery; nanomagnetic gene transfection; biomagnetics; gradient magnetic field; permanent magnets magnetic nanoparticles; transfection; SH-SY5Y; hippocampal neurons; cortical neurons; non-viral gene delivery; nanomagnetic gene transfection; biomagnetics; gradient magnetic field; permanent magnets
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Subramanian, M.; Tyler, A.-J.; Luther, E.M.; Daniel, E.D.; Lim, J.; Dobson, J. Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies. Nanomaterials 2017, 7, 28.

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