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Nanomaterials 2016, 6(7), 130;

Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging

Liaoning Key Laboratory of Food Biological Technology, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan 1, Ganjingzi District, Dalian 116034, China
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
Author to whom correspondence should be addressed.
Academic Editor: Thomas Nann
Received: 18 April 2016 / Revised: 25 June 2016 / Accepted: 30 June 2016 / Published: 5 July 2016
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Fluorescent nanostructures (NSs) derived from polysaccharides have drawn great attention as novel fluorescent probes for potential bio-imaging applications. Herein, we reported a facile alkali-assisted hydrothermal method to fabricate polysaccharide NSs using starch and chitosan as raw materials. Transmission electron microscopy (TEM) demonstrated that the average particle sizes are 14 nm and 75 nm for starch and chitosan NSs, respectively. Fourier transform infrared (FT-IR) spectroscopy analysis showed that there are a large number of hydroxyl or amino groups on the surface of these polysaccharide-based NSs. Strong fluorescence with an excitation-dependent emission behaviour was observed under ultraviolet excitation. Interestingly, the photostability of the NSs was found to be superior to fluorescein and rhodamine B. The quantum yield of starch NSs could reach 11.12% under the excitation of 360 nm. The oxidative metal ions including Cu(II), Hg(II)and Fe(III) exhibited a quench effect on the fluorescence intensity of the prepared NSs. Both of the two kinds of the multicoloured NSs showed a maximum fluorescence intensity at pH 7, while the fluorescence intensity decreased dramatically when they were put in an either acidic or basic environment (at pH 3 or 11). The cytotoxicity study of starch NSs showed that low cell cytotoxicity and 80% viability was found after 24 h incubation, when their concentration was less than 10 mg/mL. The study also showed the possibility of using the multicoloured starch NSs for mouse melanoma cells and guppy fish imaging. View Full-Text
Keywords: polysaccharide nanostructures; fluorescent materials; quantum yield; cell imaging; fish imaging polysaccharide nanostructures; fluorescent materials; quantum yield; cell imaging; fish imaging

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Zu, Y.; Bi, J.; Yan, H.; Wang, H.; Song, Y.; Zhu, B.-W.; Tan, M. Nanostructures Derived from Starch and Chitosan for Fluorescence Bio-Imaging. Nanomaterials 2016, 6, 130.

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