Next Article in Journal
Synthesis of Mesoporous Metal Oxides by Structure Replication: Thermal Analysis of Metal Nitrates in Porous Carbon Matrices
Next Article in Special Issue
Surface Wettability Modification of Cyclic Olefin Polymer by Direct Femtosecond Laser Irradiation
Previous Article in Journal
Preparation and Photocatalytic Activity of Potassium- Incorporated Titanium Oxide Nanostructures Produced by the Wet Corrosion Process Using Various Titanium Alloys
Previous Article in Special Issue
Hyaluronic Acid-Chitosan Nanoparticles to Deliver Gd-DTPA for MR Cancer Imaging
Article Menu

Export Article

Open AccessArticle
Nanomaterials 2015, 5(3), 1418-1430;

Comparison of the in Vitro Uptake and Toxicity of Collagen- and Synthetic Polymer-Coated Gold Nanoparticles

Fachbereich Physik, Philipps Universität Marburg, Marburg 35037, Germany
Faculty of Physics, Babeş-Bolyai University, Cluj-Napoca 400084, Romania
CIC Biomagune, San Sebastian 20009, Spain
Dr. von Hauner Children’s Hospital, Ludwig Maximilians University, Munich 80337, Germany
Author to whom correspondence should be addressed.
Academic Editor: Subramanian Tamil Selvan
Received: 3 August 2015 / Revised: 19 August 2015 / Accepted: 20 August 2015 / Published: 27 August 2015
(This article belongs to the Special Issue Nanoparticles in Theranostics)
View Full-Text   |   Download PDF [982 KB, uploaded 27 August 2015]   |  


We studied the physico-chemical properties (size, shape, zeta-potential), cellular internalization and toxicity of gold nanoparticles (NPs) stabilized with the most abundant mammalian protein, collagen. The properties of these gold NPs were compared to the same sized gold NPs coated with synthetic poly(isobutylene-alt-maleic anhydride) (PMA). Intracellular uptake and cytotoxicity were assessed in two cell lines (cervical carcinoma and lung adenocarcinoma cells) by employing inductively-coupled plasma-mass spectrometry (ICP-MS) analysis and a cell viability assay based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), respectively. We found that the collagen-coated gold NPs exhibit lower cytotoxicity, but higher uptake levels than PMA-coated gold NPs. These results demonstrate that the surface coating of Au NPs plays a decisive role in their biocompatibility. View Full-Text
Keywords: gold nanoparticles; cellular uptake; toxicity; biocompatibility gold nanoparticles; cellular uptake; toxicity; biocompatibility

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Marisca, O.T.; Kantner, K.; Pfeiffer, C.; Zhang, Q.; Pelaz, B.; Leopold, N.; Parak, W.J.; Rejman, J. Comparison of the in Vitro Uptake and Toxicity of Collagen- and Synthetic Polymer-Coated Gold Nanoparticles. Nanomaterials 2015, 5, 1418-1430.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top