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Nanomaterials 2015, 5(2), 874-884; doi:10.3390/nano5020874

Targeting of Apoptotic Cells Using Functionalized Fe2O3 Nanoparticles

1
Department of Neurosurgery, Graduate School of Medicine, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan
2
Center of Materials Science, Zewail City of Science and Technology, Sheikh Zayed District, 12588, 6th of October City, Giza, Egypt
3
Department of Neurosurgery, Aomori Prefecture Central Hospital, 2 1 1 Higashitsukurimichi, Aomori 0308553, Japan
4
Department of Neurosurgery, Graduate School of Medicine, Akita University, 1-1-1 Hondo, Akita 010-8543, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Pablo del Pino
Received: 29 March 2015 / Accepted: 20 May 2015 / Published: 26 May 2015
(This article belongs to the Special Issue Nanoparticles in Theranostics)
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Abstract

Fe2O3 nanoparticles (NPs) have been synthesized and functionalized with SiO2 and -NH2 group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to be ca. 80% for Fe2O3-SiO-NH2 functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs) in vivo and in vitro has been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis. View Full-Text
Keywords: functionalized nanoparticles (FNPs); fluorescently-labeled poly-caspase inhibitor (SR-FLIVO); apoptosis; brain diagnosis functionalized nanoparticles (FNPs); fluorescently-labeled poly-caspase inhibitor (SR-FLIVO); apoptosis; brain diagnosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mekawy, M.; Saito, A.; Shimizu, H.; Tominaga, T. Targeting of Apoptotic Cells Using Functionalized Fe2O3 Nanoparticles. Nanomaterials 2015, 5, 874-884.

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