Targeting of Apoptotic Cells Using Functionalized Fe2O3 Nanoparticles
AbstractFe2O3 nanoparticles (NPs) have been synthesized and functionalized with SiO2 and -NH2 group, respectively. Conjugation to fluorescently-labeled poly-caspase inhibitor (SR-FLIVO) has been carried out for better cellular uptake studies of apoptosis arising from brain focal cerebral ischemia. Highest conjugation affinity to SR-FLIVO was found to be ca. 80% for Fe2O3-SiO-NH2 functionalized nanoparticles (FNPs). Tracking of SR-FLIVO conjugated functionalized nanoparticles (SR-FLIVO-FNPs) in vivo and in vitro has been carried out and detected using microscopic techniques after histochemical staining methods. Experimental results revealed that SR-FLIVO-FNPs probe could passively cross the blood brain barrier (BBB) and accumulated within the apoptotic cell. Optimization of SR-FLIVO-FNPs probe can effectively promise to open a new era for intracellular drug delivery and brain diagnosis. View Full-Text
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Mekawy, M.; Saito, A.; Shimizu, H.; Tominaga, T. Targeting of Apoptotic Cells Using Functionalized Fe2O3 Nanoparticles. Nanomaterials 2015, 5, 874-884.
Mekawy M, Saito A, Shimizu H, Tominaga T. Targeting of Apoptotic Cells Using Functionalized Fe2O3 Nanoparticles. Nanomaterials. 2015; 5(2):874-884.Chicago/Turabian Style
Mekawy, Moataz; Saito, Atsushi; Shimizu, Hiroaki; Tominaga, Teiji. 2015. "Targeting of Apoptotic Cells Using Functionalized Fe2O3 Nanoparticles." Nanomaterials 5, no. 2: 874-884.