Next Article in Journal
Quantitative Assessment of Antimicrobial Activity of PLGA Films Loaded with 4-Hexylresorcinol
Next Article in Special Issue
Methods to Improve Osseointegration of Dental Implants in Low Quality (Type-IV) Bone: An Overview
Previous Article in Journal
Influence of Chromium-Cobalt-Molybdenum Alloy (ASTM F75) on Bone Ingrowth in an Experimental Animal Model
Previous Article in Special Issue
Evaluation of PBS Treatment and PEI Coating Effects on Surface Morphology and Cellular Response of 3D-Printed Alginate Scaffolds
Article Menu
Issue 1 (March) cover image

Export Article

Open AccessFeature PaperArticle
J. Funct. Biomater. 2018, 9(1), 3; https://doi.org/10.3390/jfb9010003

The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens

1
New Jersey Center for Biomaterials, Rutgers University, 145 Bevier Rd., Piscataway, NJ 08854, USA
2
Osiris Therapeutics, Inc., Columbia, MD 21046, USA
*
Author to whom correspondence should be addressed.
Received: 7 December 2017 / Revised: 30 December 2017 / Accepted: 3 January 2018 / Published: 8 January 2018
(This article belongs to the Special Issue Journal of Functional Biomaterials: Feature Papers 2016)
Full-Text   |   PDF [2915 KB, uploaded 9 January 2018]   |  

Abstract

Biofilm, a community of bacteria, is tolerant to antimicrobial agents and ubiquitous in chronic wounds. In a chronic DFU (Diabetic Foot Ulcers) clinical trial, the use of a human cryopreserved viable amniotic membrane (CVAM) resulted in a high rate of wound closure and reduction of wound-related infections. Our previous study demonstrated that CVAM possesses intrinsic antimicrobial activity against a spectrum of wound-associated bacteria under planktonic culture conditions. In this study, we evaluated the effect of CVAM and cryopreserved viable umbilical tissue (CVUT) on biofilm formation of S. aureus and P. aeruginosa, the two most prominent pathogens associated with chronic wounds. Firstly, we showed that, like CVAM, CVUT released antibacterial activity against multiple bacterial pathogens and the devitalization of CVUT reduced its antibacterial activity. The biofilm formation was then measured using a high throughput method and an ex vivo porcine dermal tissue model. We demonstrate that the formation of biofilm was significantly reduced in the presence of CVAM- or CVUT-derived conditioned media compared to control assay medium. The formation of P. aeruginosa biofilm on CVAM-conditioned medium saturated porcine dermal tissues was reduced 97% compared with the biofilm formation on the control medium saturated dermal tissues. The formation of S. auerus biofilm on CVUT-conditioned medium saturated dermal tissues was reduced 72% compared with the biofilm formation on the control tissues. This study is the first to show that human cryopreserved viable placental tissues release factors that inhibit biofilm formation. Our results provide an explanation for the in vivo observation of their ability to support wound healing. View Full-Text
Keywords: biofilm; chronic wound; cryopreserved amniotic membrane; cryopreserved umbilical cord tissue; antibacterial biofilm; chronic wound; cryopreserved amniotic membrane; cryopreserved umbilical cord tissue; antibacterial
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Mao, Y.; Singh-Varma, A.; Hoffman, T.; Dhall, S.; Danilkovitch, A.; Kohn, J. The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens. J. Funct. Biomater. 2018, 9, 3.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top