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J. Funct. Biomater. 2012, 3(2), 298-312; doi:10.3390/jfb3020298
Article

Increasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chemistry Applied onto Inner Surface of the Titration Well

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Received: 9 March 2012 / Revised: 4 April 2012 / Accepted: 9 April 2012 / Published: 18 April 2012
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Abstract

The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false positives. This goal was obtained thanks to the association of plasma and wet chemistries applied to the inner surface of the titration well. The polypropylene surface was plasma-activated and then, dip-coated with different amphiphilic molecules. These molecules have more or less long hydrocarbon chains and may be charged. The modified surfaces were characterized in terms of hydrophilic—phobic character, surface chemical groups and topography. Finally, the coated wells were tested during the ELISA titration of the specific antibody capture of the α-synuclein protein. The highest sensitivity is obtained with polar (Θ = 35°), negatively charged and smooth inner surface.
Keywords: amphiphilic molecules; cold plasma; ELISA titration; α-synuclein protein amphiphilic molecules; cold plasma; ELISA titration; α-synuclein protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mille, C.; Debarnot, D.; Zorzi, W.; El Moualij, B.; Coudreuse, A.; Legeay, G.; Quadrio, I.; Perret-Liaudet, A.; Poncin-Epaillard, F. Increasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chemistry Applied onto Inner Surface of the Titration Well. J. Funct. Biomater. 2012, 3, 298-312.

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J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert