J. Funct. Biomater. 2012, 3(2), 298-312; doi:10.3390/jfb3020298
Increasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chemistry Applied onto Inner Surface of the Titration Well
1
Département Polymères, Institut des Molécules et Matériaux du Mans, Colloïdes et Interfaces, Lunam Université, UMR Université du Maine—CNRS No. 6283, Avenue Olivier Messiaen, Le Mans Cedex 72085, France
2
Centre de Recherche sur les Protéines Prion, Institut de Pharmacie, B36, No. 1 avenue de l’Hôpital, Liège 4000, Belgium
3
CTTM, 20 rue Thalès de Milet, Le Mans 72000, France
4
Centre Mémoire de Ressources et Recherche, Service de Neurobiologie, Groupement Hospitalier Est du CHU, 56 bd Pinel, Bron cedex 69677, France
*
Author to whom correspondence should be addressed.
Received: 9 March 2012 / Revised: 4 April 2012 / Accepted: 9 April 2012 / Published: 18 April 2012
Abstract
The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false positives. This goal was obtained thanks to the association of plasma and wet chemistries applied to the inner surface of the titration well. The polypropylene surface was plasma-activated and then, dip-coated with different amphiphilic molecules. These molecules have more or less long hydrocarbon chains and may be charged. The modified surfaces were characterized in terms of hydrophilic—phobic character, surface chemical groups and topography. Finally, the coated wells were tested during the ELISA titration of the specific antibody capture of the α-synuclein protein. The highest sensitivity is obtained with polar (Θ = 35°), negatively charged and smooth inner surface.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Mille, C.; Debarnot, D.; Zorzi, W.; El Moualij, B.; Coudreuse, A.; Legeay, G.; Quadrio, I.; Perret-Liaudet, A.; Poncin-Epaillard, F. Increasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chemistry Applied onto Inner Surface of the Titration Well. J. Funct. Biomater. 2012, 3, 298-312.
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