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J. Funct. Biomater. 2012, 3(2), 313-326; doi:10.3390/jfb3020313
Article

Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC

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Received: 6 March 2012; in revised form: 10 April 2012 / Accepted: 11 April 2012 / Published: 19 April 2012
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Abstract: VEGF (vascular endothelial growth factor) promotes vascularization and remodeling of bone substitutes. The aim of this study was to examine the effect of distinct resorbable ceramic carriers on bone forming capacities of VEGF transfected bone marrow stromal cells (BMSC). A critical size defect of the radius in rabbits was filled either by a low surface scaffold called beta-TCP (tricalciumphsphate) or the high surface scaffold CDHA (calcium deficient hydroxy-apatite) loaded with autologous BMSC, which were either transfected with a control plasmid or a plasmid coding for phVEGF165. They were compared to unloaded scaffolds. Thus, six treatment groups (n = 6 in each group) were followed by X-ray over 16 weeks. After probe retrieval, the volume of new bone was measured by micro-CT scans and vascularization was assessed in histology. While only minor bone formation was found in both carriers when implanted alone, BMSC led to increased osteogenesis in both carriers. VEGF promoted vascularization of the scaffolds significantly in contrast to BMSC alone. Bone formation was increased in the beta-TCP group, whereas it was inhibited in the CDHA group that showed faster scaffold degradation. The results indicate that the interaction of VEGF transfected BMSC with resorbable ceramic carrier influences the ability to promote bone healing.
Keywords: angiogenesis; bone substitutes; VEGF; osteogenesis; BMSC angiogenesis; bone substitutes; VEGF; osteogenesis; BMSC
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Geiger, F.; Beverungen, M.; Lorenz, H.; Wieland, J.; Fehr, M.; Kasten, P. Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC. J. Funct. Biomater. 2012, 3, 313-326.

AMA Style

Geiger F, Beverungen M, Lorenz H, Wieland J, Fehr M, Kasten P. Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC. Journal of Functional Biomaterials. 2012; 3(2):313-326.

Chicago/Turabian Style

Geiger, Florian; Beverungen, Mirjam; Lorenz, Helga; Wieland, Julia; Fehr, Michael; Kasten, Philip. 2012. "Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC." J. Funct. Biomater. 3, no. 2: 313-326.


J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert