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Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC
Division of Experimental Orthopedics, Orthopedic University Hospital of Heidelberg, Heidelberg 69118, Germany
Department of Spine Surgery, Orthopedic University Hospital Friedrichsheim, J.W. Goethe University of Frankfurt, Frankfurt, Main 60528, Germany
University of Veterinary Medicine Hannover, Hannover 30559, Germany
Harlan Cytotest Cell Research GmbH, Rossdorf 64380, Germany
Department of Orthopedic Surgery, University of Dresden, Dresden 01307, Germany
* Author to whom correspondence should be addressed.
Received: 6 March 2012; in revised form: 10 April 2012 / Accepted: 11 April 2012 / Published: 19 April 2012
Abstract: VEGF (vascular endothelial growth factor) promotes vascularization and remodeling of bone substitutes. The aim of this study was to examine the effect of distinct resorbable ceramic carriers on bone forming capacities of VEGF transfected bone marrow stromal cells (BMSC). A critical size defect of the radius in rabbits was filled either by a low surface scaffold called beta-TCP (tricalciumphsphate) or the high surface scaffold CDHA (calcium deficient hydroxy-apatite) loaded with autologous BMSC, which were either transfected with a control plasmid or a plasmid coding for phVEGF165. They were compared to unloaded scaffolds. Thus, six treatment groups (n = 6 in each group) were followed by X-ray over 16 weeks. After probe retrieval, the volume of new bone was measured by micro-CT scans and vascularization was assessed in histology. While only minor bone formation was found in both carriers when implanted alone, BMSC led to increased osteogenesis in both carriers. VEGF promoted vascularization of the scaffolds significantly in contrast to BMSC alone. Bone formation was increased in the beta-TCP group, whereas it was inhibited in the CDHA group that showed faster scaffold degradation. The results indicate that the interaction of VEGF transfected BMSC with resorbable ceramic carrier influences the ability to promote bone healing.
Keywords: angiogenesis; bone substitutes; VEGF; osteogenesis; BMSC
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Geiger, F.; Beverungen, M.; Lorenz, H.; Wieland, J.; Fehr, M.; Kasten, P. Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC. J. Funct. Biomater. 2012, 3, 313-326.
Geiger F, Beverungen M, Lorenz H, Wieland J, Fehr M, Kasten P. Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC. Journal of Functional Biomaterials. 2012; 3(2):313-326.
Geiger, Florian; Beverungen, Mirjam; Lorenz, Helga; Wieland, Julia; Fehr, Michael; Kasten, Philip. 2012. "Bone Substitute Effect on Vascularization and Bone Remodeling after Application of phVEGF165 Transfected BMSC." J. Funct. Biomater. 3, no. 2: 313-326.