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Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation
Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Giessen, Germany
Department of Chemical Engineering and Biotechnology, University of Applied Sciences, 64297 Darmstadt, Germany
Department of Chemical Engineering, Kansas State University, Manhattan, KS 66506, USA
* Author to whom correspondence should be addressed.
Received: 15 July 2011; Accepted: 4 August 2011 / Published: 8 August 2011
Abstract: For back disorders, cell therapy is one approach for a real regeneration of a degenerated nucleus pulposus. Human mesenchymal stem cells (hMSC) could be differentiated into nucleus pulposus (NP)-like cells and used for cell therapy. Therefore it is necessary to find a suitable biocompatible matrix, which supports differentiation. It could be shown that a differentiation of hMSC in a microbial transglutaminase cross-linked gelatin matrix is possible, but resulted in a more chondrocyte-like cell type. The addition of porcine NP extract to the gelatin matrix caused a differentiation closer to the desired NP cell phenotype. This concludes that a hydrogel containing NP extract without any other supplements could be suitable for differentiation of hMSCs into NP cells. The NP extract itself can be cross-linked by transglutaminase to build a hydrogel free of NP atypical substrates. As shown by side-specific biotinylation, the NP extract contains molecules with free glutamine and lysine residues available for the transglutaminase.
Keywords: nucleus pulposus extract; gelatin; mesenchymal stem cells; transglutaminase
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Salzig, D.; Schmiermund, A.; Gebauer, E.; Fuchsbauer, H.-L.; Czermak, P. Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation. J. Funct. Biomater. 2011, 2, 155-172.
Salzig D, Schmiermund A, Gebauer E, Fuchsbauer H-L, Czermak P. Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation. Journal of Functional Biomaterials. 2011; 2(3):155-172.
Salzig, Denise; Schmiermund, Alexandra; Gebauer, Elke; Fuchsbauer, Hans-Lothar; Czermak, Peter. 2011. "Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation." J. Funct. Biomater. 2, no. 3: 155-172.