Next Article in Journal
Temperature-Responsive Biocompatible Copolymers Incorporating Hyperbranched Polyglycerols for Adjustable Functionality
Previous Article in Journal / Special Issue
Controlled Positioning of Cells in Biomaterials—Approaches Towards 3D Tissue Printing
Article Menu

Export Article

Open AccessArticle
J. Funct. Biomater. 2011, 2(3), 155-172; doi:10.3390/jfb2030155

Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation

1
Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Giessen, Germany
2
Department of Chemical Engineering and Biotechnology, University of Applied Sciences, 64297 Darmstadt, Germany
3
Department of Chemical Engineering, Kansas State University, Manhattan, KS 66506, USA
*
Author to whom correspondence should be addressed.
Received: 15 July 2011 / Accepted: 4 August 2011 / Published: 8 August 2011
(This article belongs to the Special Issue Stem Cells and Biomaterials)
View Full-Text   |   Download PDF [487 KB, uploaded 8 August 2011]   |  

Abstract

For back disorders, cell therapy is one approach for a real regeneration of a degenerated nucleus pulposus. Human mesenchymal stem cells (hMSC) could be differentiated into nucleus pulposus (NP)-like cells and used for cell therapy. Therefore it is necessary to find a suitable biocompatible matrix, which supports differentiation. It could be shown that a differentiation of hMSC in a microbial transglutaminase cross-linked gelatin matrix is possible, but resulted in a more chondrocyte-like cell type. The addition of porcine NP extract to the gelatin matrix caused a differentiation closer to the desired NP cell phenotype. This concludes that a hydrogel containing NP extract without any other supplements could be suitable for differentiation of hMSCs into NP cells. The NP extract itself can be cross-linked by transglutaminase to build a hydrogel free of NP atypical substrates. As shown by side-specific biotinylation, the NP extract contains molecules with free glutamine and lysine residues available for the transglutaminase. View Full-Text
Keywords: nucleus pulposus extract; gelatin; mesenchymal stem cells; transglutaminase nucleus pulposus extract; gelatin; mesenchymal stem cells; transglutaminase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Salzig, D.; Schmiermund, A.; Gebauer, E.; Fuchsbauer, H.-L.; Czermak, P. Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation. J. Funct. Biomater. 2011, 2, 155-172.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top