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J. Funct. Biomater. 2011, 2(3), 155-172; doi:10.3390/jfb2030155
Article

Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation

1,* , 1
,
2
,
2
 and
1,3
1 Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstraße 14, 35390 Giessen, Germany 2 Department of Chemical Engineering and Biotechnology, University of Applied Sciences, 64297 Darmstadt, Germany 3 Department of Chemical Engineering, Kansas State University, Manhattan, KS 66506, USA
* Author to whom correspondence should be addressed.
Received: 15 July 2011 / Accepted: 4 August 2011 / Published: 8 August 2011
(This article belongs to the Special Issue Stem Cells and Biomaterials)
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Abstract

For back disorders, cell therapy is one approach for a real regeneration of a degenerated nucleus pulposus. Human mesenchymal stem cells (hMSC) could be differentiated into nucleus pulposus (NP)-like cells and used for cell therapy. Therefore it is necessary to find a suitable biocompatible matrix, which supports differentiation. It could be shown that a differentiation of hMSC in a microbial transglutaminase cross-linked gelatin matrix is possible, but resulted in a more chondrocyte-like cell type. The addition of porcine NP extract to the gelatin matrix caused a differentiation closer to the desired NP cell phenotype. This concludes that a hydrogel containing NP extract without any other supplements could be suitable for differentiation of hMSCs into NP cells. The NP extract itself can be cross-linked by transglutaminase to build a hydrogel free of NP atypical substrates. As shown by side-specific biotinylation, the NP extract contains molecules with free glutamine and lysine residues available for the transglutaminase.
Keywords: nucleus pulposus extract; gelatin; mesenchymal stem cells; transglutaminase nucleus pulposus extract; gelatin; mesenchymal stem cells; transglutaminase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Salzig, D.; Schmiermund, A.; Gebauer, E.; Fuchsbauer, H.-L.; Czermak, P. Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation. J. Funct. Biomater. 2011, 2, 155-172.

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