Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation
AbstractFor back disorders, cell therapy is one approach for a real regeneration of a degenerated nucleus pulposus. Human mesenchymal stem cells (hMSC) could be differentiated into nucleus pulposus (NP)-like cells and used for cell therapy. Therefore it is necessary to find a suitable biocompatible matrix, which supports differentiation. It could be shown that a differentiation of hMSC in a microbial transglutaminase cross-linked gelatin matrix is possible, but resulted in a more chondrocyte-like cell type. The addition of porcine NP extract to the gelatin matrix caused a differentiation closer to the desired NP cell phenotype. This concludes that a hydrogel containing NP extract without any other supplements could be suitable for differentiation of hMSCs into NP cells. The NP extract itself can be cross-linked by transglutaminase to build a hydrogel free of NP atypical substrates. As shown by side-specific biotinylation, the NP extract contains molecules with free glutamine and lysine residues available for the transglutaminase.
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Salzig, D.; Schmiermund, A.; Gebauer, E.; Fuchsbauer, H.-L.; Czermak, P. Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation. J. Funct. Biomater. 2011, 2, 155-172.
Salzig D, Schmiermund A, Gebauer E, Fuchsbauer H-L, Czermak P. Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation. Journal of Functional Biomaterials. 2011; 2(3):155-172.Chicago/Turabian Style
Salzig, Denise; Schmiermund, Alexandra; Gebauer, Elke; Fuchsbauer, Hans-Lothar; Czermak, Peter. 2011. "Influence of Porcine Intervertebral Disc Matrix on Stem Cell Differentiation." J. Funct. Biomater. 2, no. 3: 155-172.