Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated Chitooligosaccharides on the Osteosarcoma MG63 Cell Line
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Synthesis of P-COS
2.3. Characterization
3. Results and Discussion
3.1. General description
3.2. Solubility of P-COS
Solvent | Chitooligosaccharides | P-Chitooligosaccharides |
---|---|---|
H2O | Soluble | Soluble |
Acetic acid (1%) | Soluble | Soluble |
NaOH (1%) | Soluble | Soluble |
HCl (1%) | Soluble | Soluble |
Dimethyl sulfoxide | Soluble | Soluble |
Dimethyl acetamide | Insoluble | Insoluble |
Pyridine | Swelling | Swelling |
Dimethyl formamide | Insoluble | Insoluble |
Ethanol | Insoluble | Insoluble |
Acetone | Insoluble | Insoluble |
Chloroform | Insoluble | Insoluble |
3.3. Stretching frequency P-COS
3.4. Thermal stability of P-COS
3.5. 13C NMR spectra of P-chitooligosaccharide
3.6. 31P NMR spectra of P-chitooligosaccharide
3.7. Biocompatibility of P-COS in an osteosarcoma–cell line
3.8. ALP activity of phosphorylated chitooligosaccharides
4. Conclusions
Acknowledgements
References
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Venkatesan, J.; Pangestuti, R.; Qian, Z.-J.; Ryu, B.; Kim, S.-K. Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated Chitooligosaccharides on the Osteosarcoma MG63 Cell Line. J. Funct. Biomater. 2010, 1, 3-13. https://doi.org/10.3390/jfb1010003
Venkatesan J, Pangestuti R, Qian Z-J, Ryu B, Kim S-K. Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated Chitooligosaccharides on the Osteosarcoma MG63 Cell Line. Journal of Functional Biomaterials. 2010; 1(1):3-13. https://doi.org/10.3390/jfb1010003
Chicago/Turabian StyleVenkatesan, Jayachandran, Ratih Pangestuti, Zhong-Ji Qian, BoMi Ryu, and Se-Kwon Kim. 2010. "Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated Chitooligosaccharides on the Osteosarcoma MG63 Cell Line" Journal of Functional Biomaterials 1, no. 1: 3-13. https://doi.org/10.3390/jfb1010003